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Tonbo Biosciences/APC Anti-Human CD8a (Hit8a)/20-0089-T025 /25 tests

TheHit8aantibodyisspecificforthe32-34kDaalphachainofhumanCD8,knownasCD8aorCD8alpha.CD8acanformahomodimer(CD8alpha-alpha),butismorecommonlyexpressedasaheterodimerwithasecondchainknownasCD8borCD8beta.CD8actsasaco-receptorforantigenrecognitionandsubsequentTcellactivationthatisinitiateduponbindingoftheTcellreceptor(TCR)toantigen-bearingMHCClassImolecules.ThecytoplasmicdomainsofCD8providebindingsitesforthetyrosinekinaselck,facilitatingintracellularsignalingeventsthatleadtoTcellactivation,development,andcytotoxiceffectorfunctions.CD8+cytotoxicTcells(CTLs)playanimportantroleininducingcelldeathoftumorcells,aswellascellsinfectedbyvirus,bacteriaorparasites.

TheHit8aantibodyiswidelyusedasaphenotypicMarkerforCD8oncytotoxicTcells,thymocytes,aswellasoncertaincelltypesthatdonotalsoexpresstheTCR,includingsomeNKcellsandlymphoiddendriticcells.IfusedtogetherwithanalternativeAnti-HumanCD8acloneRPA-T8,theHit8aantibodywillnotblockbindingofRPA-T8toCD8a.

NameAPCAnti-HumanCD8a(Hit8a)
Cat.No.20-0089
AlternativeNamesCD8alpha,leu-2a
GeneID925
CloneHit8a
IsotypeMouseIgG1,kappa
ReactivityHuman
CrossReactivityChimpanzee,Rhesus
FormatAPC
ApplicationsFlowCytometry
Citations*

CoppietersKT,DottaF,AmirianN,CampbellPD,KayTWH,AtkinsonMA,RoepBOandvonHerrathMG.2012.J.Exp.Med.109:51-60.(insitutetramerstaining/Immunohistochemistry–frozentissue)

KumariA,BrendelC,HochlausA,NeubauerA,andBurchertA.2012.Blood.119:530-539.(Flowcytometry)

KryczekI,BanerjeeM,ChengP,VatanL,etal.2009.Blood.114:1141–1149.(Immunofluorescencemicroscopy)

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