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The145-2C11antibodyisspecificformouseCD3e,alsoknownasCD3epsilon,a20kDasubunitoftheTcellreceptorcomplex,alongwithCD3gammaandCD3delta.TheseintegralmembraneproteinchainsassemblewithadditionalchainsoftheTcellreceptor(TCR),aswellasCD3zetachain,toformtheTcellreceptor�CD3complex.Togetherwithco-receptorsCD4orCD8,thecomplexservestorecognizeantigensboundtoMHCmoleculesonantigen-presentingcells.SuchinteractionspromoteTcellreceptorsignaling(Tcellactivation)andcanresultinanumberofcellularresponsesincludingproliferation,differentiation,productionofcytokinesoractivation-inducedcelldeath.CD3isdifferentiallyexpressedduringthymocyte-to-TcelldevelopmentandonallmatureTcells.
The145-2C11antibodyisawidelyusedphenotypicMarkerformouseTcells.Inaddition,bindingof145-2C11antibodytoCD3ecaninducecellactivation.ArecentpublicationofthecrystalstructureofamurineCD3e-mitogenicantibodycomplexprovidesfurtherinsightintotheactionofcommonlyusedagonistantibodies(Fernandes,R.A.etal.2012.J.Biol.Chem.287:13324-13335).
| Name | APC-Cyanine7Anti-MouseCD3e(145-2C11) |
|---|---|
| Cat.No. | 25-0031 |
| AlternativeNames | CD3epsilon |
| GeneID | 12501 |
| Clone | 145-2C11 |
| Isotype | ArmenianHamsterIgG |
| Reactivity | Mouse |
| CrossReactivity | No |
| Format | APC-Cyanine7 |
| Applications | FlowCytometry |
| Citations* | StaehliR,LudigsK,HeinzLX,Sequin-EstevezQ,FereroI,BraunM,SchroderK,RebsamenM,TardivelA,MattmannC,MacDonaldHR,RomeroP,ReithW,GuardaG,andTschoppJ.2012.J.Immunol.188:3820-3828.(invitroactivation) TodoT,WuG,ChaiNN,HeY,MartinsG,GuptaA,FairJ,LiuNY,JordanS,andKleinA.2012.Int.Immunol.10:1093.(invivoassay) MiraE,LeonB,BarberDF,Jimenez-BarandaS,GoyaI,AlmonacidL,MarquezG,ZaballosA,MartinezC,SteinJV,ArdavinCandManesS.2012.J.Immunol.181(5):3524-3534.(invitroactivation,Immunohistochemistry–frozentissue) Becker-HermanA,Meyer-BahlburgA,SchwartzMA,JacksonSW,HudkinsKL,LiuC,SatherBD,KhimS,LiggittD,SongW,SilvermanGJ,AlpersCEandRawlingsDJ.2011.J.Exp.Med.208:2033-2042.(Immunofluorescencemicroscopy–OCTembeddedfrozentissue) SalmondRJ,FilbyA,PirinenN,MageeAI,andZamoyskaR.2010.Blood.117:108-117.(Immunoprecipitation) TilleySL,JaradatM,StapletonC,DixonD,HuaX,EriksonCJ,.McCaskillJG,ChasonKD,LiaoG,JaniaL,KollerBH,andJettenAM.2007.J.Immunol.178:3208-18.(Immunohistochemistry–frozentissue) IsakovN,WangeRL,BurgessWH,WattsJD,AebersoldR,andSamelsonLE.1995.J.Exp.Med.181:375-380.(invitroactivation,Immunoprecipitation) SalvadoriS,GansbacherB,PizzimentiAM,andZierKS.1994.J.Immunol.153:5176-5182.(WesternBlotting) LeoO,FooM,SachsD,SamuelsonL,andBluestoneJ.1987.Proc.Natl.Acad.Sci.USA.84:1374(Originationofclone145-2C11,invitroactivation,invitroblocking,Immunoprecipitation) |