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TheM1/70antibodyreactswithhumanandmouseCD11b,alsoknownasintegrinalphaM.This165-170kDacellsurfaceglycoproteinispartofafamilyofintegrinreceptorsthatmediateadhesionbetweencells(cell-cell)andcomponentsoftheextracellularmatrix,e.g.fibrinogen(cell-matrix).Inaddition,integrinsareactivesignalingreceptorswhichrecruitleukocytestoinflammatorysitesandpromotecellactivation.Complete,functionalintegrinreceptorsconsistofdistinctcombinationsofintegrinchainswhicharedifferentiallyexpressed.IntegrinalphaM(CD11b)assembleswithIntegrinbeta-2(CD18)intoareceptorknownasMacrophageAntigen-1(Mac-1)orcomplementreceptortype3(CR3).ThisreceptorbindsandinducesintracellularsignalingthroughICAM-1onendothelialcellsandcanalsofacilitateremovalofiC3bbearingforeigncells.
TheM1/70antibodyiswidelyusedasaMarkerforCD11bexpressiononmousemacrophages,granulocytes,neutrophils,andNKcells.Theantibodyisalsoreportedtobecross-reactiveforRhesusmacaqueCD11b.
RecentPublications:
Molina-SanchezP,ChevreR,RiusC,FusterJJandAndresV.2015.JMolCellCardiol.84:84-94.(FlowCytometry)
| Name | APCAnti-Human/MouseCD11b(M1/70) |
|---|---|
| Cat.No. | 20-0112 |
| AlternativeNames | Mac-1,integrinαM,CR3,ITGAM |
| GeneID | 16409/3684 |
| Clone | M1/70 |
| Isotype | RatIgG2b,kappa |
| Reactivity | Human,Mouse |
| CrossReactivity | Chimpanzee,Baboon,Cynomolgus,Rhesus |
| Format | APC |
| Applications | FlowCytometry |
| Citations* | LefortCT,RossaintJ,MoserM,PetrichBG,ZarbockA,MonkleySJ,CritchleyDR,GinsbergMH,FasslerR,andLeyK.2012.Blood.119:4275-4282.(invitroblocking) GrewalJS,PilgrimMJ,GrewalS,KasmanL,WernerP,BruortonME,LondonSD,andLondonL.2011.FASEBJ.25:1680-1696.(Immunofluorescencemicroscopy–frozentissue) KimW-K,SunY,DoH,AutissierP,HalpernEF,PiatakM,LifsonJD,BurdoTH,McGrathMS,andWilliamsK.2010.J.Leukoc.Biol.87:557-567.(FlowCytometry–Rhesusmacaque) RolandCL,DineenSP,LynnKD,SullivanLA,DellingerMT,SadeghL,SullivanJP,ShamesDS,andBrekkenRA.2009.Mol.CancerTher.8:1761-1771.(Immunofluorescencemicroscopy–frozentissue) SorgH,LorchB,JasterR,FitznerB,IbrahimS,HolzhueterS,NizzeH,andVollmarB.2008.Am.J.Physio.Gastrointest.LiverPhysiol.295:G1274-1280.(Immunohistochemistry-formalin-fixedparaffinembeddedtissue) KimDD,MiwaT,KimuraY,SchwendenerRA,vanLookerenCampagneM,andSongW-C.2008.Blood.112:1109-1119.(invivoblocking) OuR,ZhangM,HuangL,FlavellRA,KoniPA,andMoskophidisD.2008.J.Virol.82:2952-2965.(Immunohistochemistry–OCTembeddedfrozentissue) NuttSL,MetcalfD,D’AmicoA,PolliM,andWuL.2005.J.Exp.Med.201:221-231.(Immunomagneticbeaddepletion) WhitelandJL,NichollsSM,ShimeldC,EastyDL,WilliamsNA,andHillTJ.1995.J.Histochem.Cytochem.43:313-320.(Immunohistochemistry–frozentissue,paraffinembeddedtissue) MillerLJ,SchwartingR,andSpringerTA.1986.J.Immunol.137:2891-2900.(Immunoprecipitation) |