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TheSK1antibodyisspecificforthe32-34kDaalphachainofhumanCD8,knownasCD8aorCD8alpha.CD8acanformahomodimer(CD8alpha-alpha),butismorecommonlyexpressedasaheterodimerwithasecondchainknownasCD8borCD8beta.CD8actsasaco-receptorforantigenrecognitionandsubsequentTcellactivationthatisinitiateduponbindingoftheTcellreceptor(TCR)toantigen-bearingMHCClassImolecules.ThecytoplasmicdomainsofCD8providebindingsitesforthetyrosinekinaselck,facilitatingintracellularsignalingeventsthatleadtoTcellactivation,development,andcytotoxiceffectorfunctions.CD8+cytotoxicTcells(CTLs)playanimportantroleininducingcelldeathoftumorcells,aswellascellsinfectedbyvirus,bacteriaorparasites.
TheSK1antibodyiswidelyusedasaphenotypicMarkerforCD8oncytotoxicTcells,thymocytes,aswellasoncertaincelltypesthatdonotalsoexpresstheTCR,includingsomeNKcellsandlymphoiddendriticcells.Itiscross-reactivewithCD8inseveralnon-humanspecies,includingBaboon,Chimpanzee,CynomolgusandRhesus.IfusedtogetherwithanalternativeAnti-HumanCD8aclone,RPA-T8,theSK1antibodywillnotblockbindingofRPA-T8toCD8a.
| Name | PerCP-Cyanine5.5Anti-HumanCD8(SK1) |
|---|---|
| Cat.No. | 65-0087 |
| AlternativeNames | CD8alpha,leu-2a |
| GeneID | 925 |
| Clone | SK1 |
| Isotype | MouseIgG1,kappa |
| Reactivity | Human |
| CrossReactivity | AfricanGreen,Baboon,Chimpanzee,Cynomolgus,Rhesus,PigtailedMacaque,SootyMangabey |
| Format | PerCP-Cyanine5.5 |
| Applications | FlowCytometry |
| Citations* | CampanelliR,PalermoB,GarbelliS,MantovaniS,LucchiP,NeckerA,LantelmeEandGiachinoC.2002.IntImmunol.14(1):39-44.(FlowCytometry) WalterS,HerrgenL,SchoorO,JungG,WernetD,BuhringH-J,RammenseeH-GandStevanovicS.2003.J.Immunol.171(10):4974-4978.(FlowCytometry) CervasiB,PaiardininM,SerafiniS,FraternaleA,MenottaM,EngramJ,LawsonB,StapransI,PiedimonteG,PernoCF,SilvestriGandMagnaniM.2006.J.Virol.80(21):10335-10345.(FlowCytometry–SootyMangabeys) VerstrepenBE,VerschoorEJ,FagrouchZC,MooijP,deGrootNG,BontropRE,BogersWM,HeeneyJLandKoopmanG.2014.PLoSONE9(4):e95103.doi:10.1371/journal.pone.0095103.(FlowCytometry–Chimpanzee) PermarSR,KlumppSA,MansfieldKG,KimW-K,GorgoneDA,LiftonMA,WilliamsKC,SchmitzJE,ReimannKA,AxthelmMK,PolackFP,GriffinDEandLetvinNL.2003.J.Virol.77(7):4396-4400.(FlowCytometry–Rhesus) AhmedAFK,OhtaniH,NioM,FuankiN,ShimaokaS,NaguraHandOhiR.2001.J.Pathol.193(3):383-389.(Immunohistochemistry) Bukowska-StrakováK,BaranJ,GawlickaMandKowalczyk,D.2006.FoliaHistochemicaetCytobiologica44(3):179-183. |