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Worthington/Elastase/LS006363/5 mg

Elastase,PurifiedChromatographicallypurified.Alyophilizedpowder.Storeat2-8°C.REQUIRESSPECIALSHIPPING:ICEPACK≥8unitspermgproteinSource:PorcinePancreas

I.U.B.:3.4.21.36

Porcinepancreaticelastasehasamolecularweightof25.9kDa,andapHoptimumof8.5. Whileelastasewillhydrolyzeawidevarietyofproteinsubstrates,itisuniqueamongproteasesinitsABIlitytohydrolyzenativeelastin,asubstratenotattackedbytrypsin,chymotrypsinorpepsin.Soybeantrypsininhibitorandkallikreininhibitorsuppressproteolyticbutnotelastolyticactivity. ElastaseisassayedusingamethodadaptedfromthatofFeinsteinetal.,Biochem.Biophys.Res.Comm.,50,1020(1973)andusingthemoresolublesubstrateofBiethetal.,Biochem.Med.,11,350(1974).

Stability/Storage:ElastaseisunstableatpH≤3.5.Whenstoredasadrypowdertheenzymeisstablefor6-12monthsat2-8°C. Elastaseproductcodes:ESandESLhavepoorsolubilityatneutralpHandatconcentrationsgreaterthan0.25%. ItishelpfultomakeprimarysolutionsinKCloralkalinebuffersbeforedilutingintothereactionmixturesormedia,compensatingforionicstrengthorpHchanges. StableatpH4.0-10.4.

TechnicalNotes:1SucAla3NAunitisapproximatelyequivalentto6elastindigestionunits.AqueousliquidsUSPensionsshouldbeasepticallyhandledtoavoidbacterialcontamination.Duetotheviscousnatureoftheaqueoussuspension(Code:ES)thevialshouldberinsedtorecovercontents.

UnitDefinition:OneUnitcleavesonemicromoleofN-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilideperminuteat25°C,pH8.0.

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