I.U.B.:1.1.1.51and1.1.1.50
Hydroxysteroiddehydrogenasescatalyzetheinterconversionofhydroxylandcarboxylgroupsofsteroids.P.testosteroniderivedhydroxysteroiddehydrogenasesareoftwotypes:3-α-hydroxysteroiddehydrogenase(αenzyme)and3-β-hydroxysteroiddehydrogenase(βenzyme).Theαenzymehasamolecularweightof47,000daltons.Theαenzymeoxidizesonly3-α-hydroxysteroidsoftheC19,C21andC24series.Itisinhibitedbyheavymetalsandsulfhydryl-bindingagents.Theβenzymecatalyzestheoxidationof3-β-hydroxysteroidsoftheC19andC21series,17-β-hydroxysteroidsoftheC18,C19andC21series,aswellascertain16-β-hydroxysteroids.Itisinhibitedbyheavymetalsandreducingagents.Theoxidationoftestosteroneisinhibitedby3,17-α-estrADIolandother1,3,5-estradienederivatives.Worthingtonsuppliestwopreparations:onefromtheregularP.testosteroni(ATCC11966)culturewhichproducesboththeαandtheβenzymes,andasecondfromamutantstrainwhichproducesalmostexclusivelytheαenzyme.Byusingboth,theβ-hydroxysteroidcanbedeterminedbydifference.
TechnicalNote:STDHPandSTDHcontainbothαandβactivities.STDHMP,however,containsonlyαactivity.
UnitDefinition:OneUnitreducesonemicromoleofNADperminuteat25°C,pH9.0usingandrosteroneortestosteroneassubstrate.
matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY
intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15
goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP
novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg
matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB
intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published)/LUCNA-1G/1 g