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Axolbio/Neural Maintenance-XF Medium/ax0032-500/1 Ea

Axol"sNeuralMaintenance-XFMediumisafullydefined,xeno-freecellculturemediumspeciallyformulatedforoptimalmaintenanceofneuralstemcellsandneuralcelltypes.Themaintenancemediumsupportsaxonalelongation,synapticactivityandreducesneuronalclumpingmakingitsuitableforlong-termcultureofneurons.

Description:

Next-generationneuralcellculturemediumrestrictedtoanimal-freecomponents.Ithasbeenoptimizedtosupportlowdensitycultureandlong-termfunctionalmaturation.

Xeno-FreeCellCultureMediumforoptimalmaintenanceof
humanneuralstemcellsandneuralcelltypes

Forlong-termneuronalcultures,Axol"s NeuralMaintenance-XFMedium providesanidealenvironmenttosupportaxonalelongationandsynapticactivity.NeuralStemCellsculturedinthismediummaintainthecapacitytodifferentiateintoneuronalandgliallineages.Themediumiscompletelydefinedensuringlot-to-lotconsistencyandreliableperformancebetweenexperiments.NeuralMaintenance-XFMediumcanmaintainhealthyneuronswhenculturedatlowdensity(10,000cells/cm2).

Odoo - Sample 1 for three columns
NeuralStemCells wereculturedona SureBond+ReadySet coatedculturevesselusing NeuralDifferentiation-XFMedium. 54hafterthawing,theNSCscellswereswitched to NeuralMaintenance-XFMedium.Theresultingneuronswerestainedfor TUJ1 expressionandcounterstainedusing DAPI. ImagetakenatDay14.
Odoo - Sample 2 for three columns

NeuralStemCells wereculturedona SureBond+ReadySet coatedculturevesselusing NeuralDifferentiation-XFMedium. 54hafterthawing,theneuralstemcellswereswitched to NeuralMaintenance-XFMediumTheresultingneuronswerestainedusinganantibodytargetingMAP2.ThecellswerecounterstainedusingDAPI.ImagetakenatDay14.

Odoo - Sample 3 for three columns


NeuralStemCells
 werebrieflyexpandedinNeuralExpansion-XFMedium andpassagedontoa SureBond+ReadySet coatedculturevessel.Thecellsweremaintainedin NeuralMaintenance-XFMedium.Thecellswerecounterstainedusing DAPI.Neuralstemcellscanbevisualizedas Ki67-positivecells.NeuronsdifferentiatedfromtheneuralstemcellsareshownusingtheMarker TUJ1.ImagetakenatDay7. 

ProductSpecification:

Size500mL
ShippingconditionsDryice
Storageconditions-80°C
XenofreeYes
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