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Everest Biotech/Monoclonal Anti-α-Adaptin 100μg/vial/2005031001/100μg/vial

Clone
Ada-1
Size
100μg/vial
Form
lyophilized
Igtype
mouseIgG2a
Specificity
Nocrossreactivitywithotherproteins.
Species
Human,rat
Immunogen
AP-2adaptorpolypeptidesfrombovinebrain.
Contents
Mouseascitesfluid,1.2%sodiumacetate,2mgBSA,with0.01mgNaN3as
preservative.

WesternblotAt2-4μg/mlwiththeappropriatesystemtodetectα-adaptinincellsandtissues.
Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyendusers.

Reconstitution:1.2%sodiumacetateorneutralPBS.If1mlofPBSisused,theantibodyconcentrationwillbe100μg/ml.
Storage:At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforalongertime.Avoidrepeatedfreezingandthawing.

1.Robinson,M.S.:CloningofCDNAsencodingtworelated100-kDcoatedvesicleproteins(alpha-adaptins).J.CellBiol.108:833-42,1989.
2.Waelter,S.;Scherzinger,E.;Hasenbank,R.;Nordhoff,E.;Lurz,R.;Goehler,H.;Gauss,C.;Sathasivam,K.;Bates,G.P.;Lehrach,H.;Wanker,E.E.:ThehuntingtininteractingproteinHIP1isaclathrinandalpha-adaptin-bindingproteininvolvedinreceptor-mediatedendocytosis.Hum.Molec.Genet.10:1807-1817,2001.

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