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SKU#: 2101291001
Lot No. 03A01
Ig type: rabbit IgG
Size: 100μg/vial
Specificity: Human, rat, mouse. No cross reactivity with other proteins.
Immunogen: A synthetic peptide corresponding to a sequence near the C-terminal of human Annexin V, identical to the related rat and mouse sequence.
Purity: Immunogen affinity purified.
Applications:
Western blot: At 1-2μg/ml with the appropriate system to detect c Annexin V in cells and tissues.
Immunohistochemistry (P): At 1-2μg/ml to detect Annexin V in formalin fixed and paraffin embedded tissues. Digesting the sections is required.
Immunocytochemistry Suitable
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg, Thimerosal, 0.05mg NaN3.
Reconstitution: 0.2ml of distilled water will yield a concentration of 500μg/ml.
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for longer time.
1. Kaplan, R.; Jaye, M.; Burgess, W. H.; Schlaepfer, D.D.; Haigler, H. T. : Cloning and expression of
cDNA for human endonexin II, a Ca(2+) and phospholipid binding protein. J. Biol. Chem. 263
2. Rodriguez-Garcia, M. I.; Kozak, C. A.; Morgan, R. O.; Fernandez, M. P. : Mouse annexin V
chromosomal localization, cDNA sequence conservation, and molecular evolution. Genomics 31:
151-157, 1996.
3. Grundmann, U.; Abel, K.-J.; Bohn, H.; Lobermann, H.; Lottspeich, F.; Kupper, H. : Characterization of
cDNA encoding human placental anticoagulant protein (PP4): homology with the lipocortin family. Proc.
Nat. Acad. Sci. 85: 3708-3712, 1988.
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