Reachbio/ColonyGEL 1101 – Human Base Medium/1101/90 mL

Product Description

ColonyGEL™ 1101   Human Base Medium

Methylcellulose-Based Medium for Colony Forming Cell Assays

Product Description

ColonyGEL™ 1101 is a base methylcellulose medium containing optimized components for colony forming cell (CFC) assays.  The base medium is designed to allow individual researchers to customize the medium by adding cytokines, conditioned media and other growth factors and supplements specific to their research.

The CFC assay is an in vitro quantitative assay used to determine the frequency of hematopoietic progenitors based on their ability to form unique, morphologically distinctive colonies in semi-solid media.

Recommended Use

Depending on the cytokine combination added, this medium can support the growth of myeloid, erythroid and mixed lineage progenitor-derived colonies such as CFU-GM, CFU-G, CFU-M, BFU-E, CFU-E, CFU-GEMM from human bone marrow (BM), cord blood (CB), mobilized peripheral blood (MPB) and peripheral blood (PB).

Product Formulation

• Methylcellulose in IMDM
• Pre-screened Fetal Bovine Serum
• Pre-screened Bovine Serum Albumin
• L-Glutamine
• 2-Mercaptoethanol

Stability and Storage

Store the entire bottle of media or aliquots at -20°C.  The product is stable at -20°C for up to 1 year from the shipment date.

Intended Use

FOR IN VITRO RESEARCH USE ONLY

Additional Information