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Reachbio/Human Peripheral Blood CD19+ B Cells/0519-101/10 million

ProductDescription

HumanPeripheralBloodCD19+BCells

 

CellEnrichment

NegativeSelection
NegativelyselectedCD19+BCellsareenrichedfromhumanperipheralblood(PB)mononuclearcells(MNCs)usinganimmunomagneticcellseparationmethodthatinvolvesanantibodycocktailthattargetsanddepletesnon-CD19+cells. 
PositiveSelection
PositivelyselectedCD19+BCellsarepurifiedfromhumanperipheralblood(PB)mononuclearcells(MNCs)usinganimmunomagneticcellseparationmethodthatinvolvesalabeledantibodythattargetstheCD19antigen.

 

SampleCollectionandProcessing

Peripheralbloodcellsarecollectedfromnormalhumanvolunteersvialeukapheresis(COBE®SpectraMNCcollectionprotocolorequivalent).Themononuclearcell-richleukocytelayer(leukapheresisproduct)iscontinuallyaspiratedbyalowflowratecollectionpumpdirectlyintoacollectionbag.ImmunomagneticcellenrichmentofCD19+cellsisperformedwithin30hoursofcollection.DonorshavebeenscreenedandfoundtobenegativeforastandardpanelofinfectiousagentsthatatminimumincludesHIV,HBVandHCV.Dependingonthespecificlot,otherinfectiousagentsmayalsohavebeentestedfor(CertificateofAnalysisincludeslot-specifictestinginformation).

 

Purity

PurityofCD19+BCellsis>90%asmeasuredbyflowcytometricanalysis.

 

StABIlityandStorage

PeripheralbloodCD19+BCellsarecryopreservedinCryoStor®CS5,aserum-freefreezingmedium.Cellsarestableforlong-termstorageat-152oCorinthegaseousphaseofliquidnitrogen.Storageat-80oCisnotrecommended.Oncethawed,thecellsmustbeusedimmediatelyandhaveafinitelifespaninculture.

 

IntendedUse

FORINVITRORESEARCHUSEONLY.NOTINTENDEDFORHUMANORVETERINARYUSEINVIVO.NOTTOBEUSEDFORANYDIAGNOSTIC,THERAPEUTICORCLINICALAPPLICATIONSWHATSOEVER. 

AdditionalInformation

CatalogNumberSelectionTypeUnitSize

(cellspervial)

Product

InfoSheet

0519-100Negative10milliondownload
0519-101Positive10milliondownload
Weight1lbs
Species

Human

TissueSource

PeripheralBlood

CellType

BCells

SelectionType

Negative,Positive

IdentifyingSurfaceMarker

CD19

UnitSize(millioncells/vial)

10million

Format

Cryopreserved

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