Reachbio/Human Peripheral Blood Mononuclear Cells (PBMCs) – Light Density/0500401/10 million,

Product Description

Human Peripheral Blood Mononuclear Cells (PBMCs) – Light Density

CatalogNumber	SelectionType	Unit Size (cells per vial)	Product Info Sheet
0500-101	none	10 million	download
0500-201	none	25 million	download
0500-301	none	50 million	download
0500-401	none	100 million	download

 

Cell Enrichment

Ficoll Density Centrifugation

Human light density peripheral blood mononuclear cells (PBMCs) are obtained by density centrifugation (Ficoll) of a leukapheresis product.

Sample Collection and Processing

Peripheral blood cells are collected from normal human volunteers via leukapheresis (COBE^® Spectra MNC collection protocol or equivalent).The mononuclear cell-rich leukocyte layer (leukapheresis product) is continually aspirated by a low flow rate collection pump directly into a collection bag. MNCs are then further enriched by density centrifugation.
Peripheral blood cells are processed within 24-30 hours of collection.Donors have been screened and found to be negative for a standard panel of infectious agents that at minimum includes HIV, HBV, and HCV. Depending on the specific lot, other infectious agents may also have been tested for (Certificate of Analysis includes lot-specific testing information).

 

Stability and Storage

Peripheral blood mononuclear cells (PBMCs) are cryopreserved in CryoStor® CS5, a serum-free freezing medium.Cells are stable for long-term storage at -152^oC or in the gaseous phase of liquid nitrogen.Storage at -80^oC is not recommended. Once thawed, the cells must be used immediately and have a finite life span in culture.

 

Intended Use

FOR IN VITRO RESEARCH USE ONLY.NOT INTENDED FOR HUMAN OR VETERINARY USE IN VIVO.NOT TO BE USED FOR ANY DIAGNOSTIC, THERAPEUTIC OR CLINICAL APPLICATIONS WHATSOEVER. 

Additional Information

Weight	1 lbs
Species	Human
Tissue Source	Peripheral Blood
Cell Type	Mononuclear Cells (MNCs)
Selection Type	Ficoll
Identifying Surface Marker	n/a
Unit Size (million cells/vial)	10 million, 100 million, 25 million, 50 million