Reachbio/Human Bone Marrow MNCs/0300300/15 million, 25 million, 5 million

Product Description

REACHBIO QUALIFIED™

Human Bone Marrow Mononuclear Cells (MNCs)

CatalogNumber	SelectionType	Unit Size (cells per vial)	Product Info Sheet
0300-100	Ficoll	5 million	download
0300-200	Ficoll	15 million	download
0300-300	Ficoll	25 million	download

 

Sample Collection and Processing

Human bone marrow (BM) MNCs are obtained by density centrifugation of BM cells.  BM cells are drawn from the posterior iliac crest of normal human donors using a syringe containing anticoagulant. BM cells are processed within 24-30 hours after collection.Donors have been screened to be negative for HIV, HBV, and HCV (see Certificate of Analysis).

 

Pre-Qualified for CFC Assays

These BM MNCs are functionally pre-qualified in a colony-forming cell (CFC) assay using ReachBio’s ColonyGEL™ methylcellulose-based media.  The frequency of myeloid and erythroid progenitors, as well as recommended plating concentration for the CFC assay, are provided for each batch of these REACHBIO QUALIFIED™ cells (see Certificate of Analysis).

 

Stability and Storage

BM mononuclear cells are cryopreserved in CryoStor® CS5, a serum-free freezing medium.Cells are stable for long-term storage at -152^oC or in the gaseous phase of liquid nitrogen.Storage at -80^oC is not recommended.  Once thawed, the cells must be used immediately and have a finite life span in culture.

 

Intended Use

FOR IN VITRO RESEARCH USE ONLY.NOT INTENDED FOR HUMAN OR VETERINARY USE IN VIVO.NOT TO BE USED FOR ANY DIAGNOSTIC, THERAPEUTIC OR CLINICAL APPLICATIONS WHATSOEVER. 

Additional Information

Weight	1 lbs
Species	Human
Tissue Source	Bone Marrow
Cell Type	Mononuclear Cells (MNCs)
Selection Type	n/a
Unit Size (million cells/vial)	15 million, 25 million, 5 million
Qualification	REACHBIO QUALIFIED
Format	Cryopreserved