Reachbio/Human Peripheral Blood CD14+ Monocytes/0514100/10 million, 20 million

Product Description

Human Peripheral Blood CD14⁺ Monocytes

CatalogNumber	SelectionType	Unit Size (cells per vial)	Product Info Sheet
0514-100	Negative	10 million	download
0514-101	Positive	10 million	download
0514-201	Positive	20 million	download

 

Cell Enrichment

Negative  Selection

Negatively selected CD14⁺ Monocytes are enriched from human peripheral blood (PB) mononuclear cells (MNCs) using an immunomagnetic cell separation method that involves an antibody cocktail that targets and depletes non-CD14⁺ cells. 

Positive Selection

Positively selected CD14⁺ Monocytes are purified from human peripheral blood (PB) mononuclear cells (MNCs) using an immunomagnetic cell separation method that involves a labeled antibody that targets the CD14 antigen.

 

Sample Collection and Processing

Peripheral blood cells are collected from normal human volunteers via leukapheresis (COBE^® Spectra MNC collection protocol or equivalent). The mononuclear cell-rich leukocyte layer (leukapheresis product) is continually aspirated by a low flow rate collection pump directly into a collection bag. Immunomagnetic cell enrichment of CD14⁺ cells is performed within 30 hours of collection.Donors have been screened and found to be negative for a standard panel of infectious agents that at minimum includes HIV, HBV and HCV. Depending on the specific lot, other infectious agents may also have been tested for (Certificate of Analysis includes lot-specific testing information).

 

Purity

Purity of CD14⁺ Monocytes is >80% (positively selected) and 80% (negatively selected) as measured by flow cytometric analysis.

 

Stability and Storage

Peripheral blood CD14⁺ monocytes are cryopreserved in CryoStor® CS5, a serum-free freezing medium.Cells are stable for long-term storage at -152^oC or in the gaseous phase of liquid nitrogen.Storage at -80^oC is not recommended. Once thawed, the cells must be used immediately and have a finite life span in culture.

 

Intended Use

FOR IN VITRO RESEARCH USE ONLY.NOT INTENDED FOR HUMAN OR VETERINARY USE IN VIVO.NOT TO BE USED FOR ANY DIAGNOSTIC, THERAPEUTIC OR CLINICAL APPLICATIONS WHATSOEVER. 

Additional Information

Weight	1 lbs
Dimensions	8 x 10 x 11 in
Species	Human
Tissue Source	Peripheral Blood
Cell Type	Monocytes
Selection Type	Negative, Positive
Identifying Surface Marker	CD14
Unit Size (million cells/vial)	10 million, 20 million
Format	Cryopreserved