Rockland/Alkaline Phosphatase Antibody/20061350100/100 µg

AlkalinePhosphataseAntibodyProperties

Description

Anti-ALKALINEPHOSPHATASE(CalfIntestine)(SHEEP)Antibody-200-6135-0100

Host

Sheep

TargetSpecies

Bovine

KnownCrossReactivity

bovine

Clonality

Polyclonal

Application

ELISA:1:4,000-1:20,000
WesternBlot:1:500-1:2,000
OtherDilution:UserOptimized

PhysicalState

Lyophilized

ShippingCondition

Ambient

Format

IgG

Label

Unconjugated

Concentration

byUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

ReconstitutionVolume

100µL

ReconstitutionBuffer

Restorewithdeionizedwater(orequivalent)

Preservative

None

Stabilizer

None

GeneName

ALP1

RelevantLinks

UniProtKB-P19111
NCBI-P19111.2
GeneID-280993

AlkalinePhosphataseAntibodyDescription

Synonyms

sheepanti-AlkalinePhosphataseAntibody,Alkalinephosphomonoesteraseantibody,ALPIantibody,Glycerophosphataseantibody,IAPantibody,Intestinalalkalinephosphataseantibody,Kasaharaisozymeantibody

Immunogen

AlkalinePhosphatase[CalfIntestine]

ImmunogenType

NativeProtein

StorageCondition

Storevialat4°Cpriortorestoration. Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.

ApplicationNote

ALKALINEPHOSPHATASEantibodyhasbeenassayedagainst1.0ugofAlkalinePhosphatase[CalfIntestine]inastandardELISAusingPeroxidaseconjugatedAffinityPurifiedanti-SheepIgG[H&L](Goat)code#611-1302and(ABTS(2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:1,000to1:5,000ofthereconstitutionconcentrationissuggestedforthisproduct.

Purity/Specifity

Anti-ALKALINEPHOSPHATASEisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-SheepSerumaswellaspurifiedandpartiallypurifiedAlkalinePhosphatase[CalfIntestine]. CrossreactivityagainstAlkalinePhosphatasefromothertissuesandspeciesmayoccurbuthavenotbeenspecificallydetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Alkaline Phosphatase Antibody/20061350100/100 µg

AlkalinePhosphataseAntibodyProperties

AlkalinePhosphataseAntibodyDescription