Rockland/Aldehyde Dehydrogenase Antibody Peroxidase Conjugated/2004344S/25 µL

AldehydeDehydrogenaseAntibodyPeroxidaseConjugatedProperties

Description

Anti-ALDEHYDEDEHYDROGENASE(Yeast)(RABBIT)AntibodyPeroxidaseConjugated-200-4344S

Host

Rabbit

TargetSpecies

Yeast

KnownCrossReactivity

Saccharomycescerevisiae(Bakersyeast)

Clonality

Polyclonal

Application

ELISA:1:5,000-1:20,000
WesternBlot:1:500-1:5,000
ImmunoPrecipitation:1:100
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Peroxidase(HorserADIsh)

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)GentamicinSulfate.DoNOTaddSodiumAzide!

StABIlizer

10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree

GeneName

ALD2,ALD5

RelevantLinks

UniProtKB-P47771
NCBI-P47771.2
GeneID-855206

AldehydeDehydrogenaseAntibodyPeroxidaseConjugatedDescription

Background

Aldehydedehydrogenaseareagroupofenzymesthatcatalysetheoxidation(dehydrogenation)ofaldehydes.AldehydedehydrogenaseisapolymorphicenzymeresponsIBLefortheoxidationofaldehydestocarboxylicacids,whichleavetheliverandaremetabolizedbythebody’smuscleandheart.ALDH2alsoplaysacrucialroleinmaintaininglowbloodlevelsofacetaldehydeduringalcoholoxidation.

Synonyms

rabbitanti-AldehydeDehydrogenaseAntibodyPeroxidaseConjugation,HRPconjugatedanti-AldehydeDehydrogenaseAntibody,Aldehydedehydrogenase[NAD(P)+]1,ALD2,ALD5,ALD

Immunogen

AldehydeDehydrogenase[Yeast]

ImmunogenType

NativeProtein

StorageCondition

Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.

ApplicationNote

Anti-Aldehydedehydrogenasehasbeenassayedagainst1.0ugofAldehydeDehydrogenase[Yeast]inastandardcaptureELISAusingABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:10,000to1:50,000ofthereconstitutionconcentrationissuggestedforthisproduct.

Purity/Specifity

AldehydedehydrogenaseisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Peroxidase,anti-RabbitSerumaswellaspurifiedandpartiallypurifiedAldehydeDehydrogenase[Yeast]. CrossreactivityagainstAldehydeDehydrogenasefromothersourcesisunknown.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Aldehyde Dehydrogenase Antibody Peroxidase Conjugated/2004344S/25 µL

AldehydeDehydrogenaseAntibodyPeroxidaseConjugatedProperties

AldehydeDehydrogenaseAntibodyPeroxidaseConjugatedDescription