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Rockland/Alcohol Dehydrogenase Antibody Biotin Conjugated/20046430100/100 µg

AlcoholDehydrogenaseAntibodyBiotinConjugatedProperties

Description
Anti-ALCOHOLDEHYDROGENASE(Yeast)(RABBIT)AntibodyBiotinConjugated-200-4643-0100
Host
Rabbit
TargetSpecies
Yeast
KnownCrossReactivity
Saccharomycescerevisiae(Bakersyeast)
Clonality
Polyclonal
Application
ELISA:1:2,000-1:15,000
WesternBlot:1:500-1:5,000
ImmunoPrecipitation:1:100
OtherDilution:UserOptimized
PhysicalState
Lyophilized
ShippingCondition
Ambient
Format
IgG
Label
Biotin
Concentration
1.0mg/mLbyUVabsorbanceat280nm
Buffer
0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2
ReconstitutionVolume
100µL
ReconstitutionBuffer
Restorewithdeionizedwater(orequivalent)
Preservative
0.01%(w/v)SodiumAzide
StABIlizer
10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree
GeneName
ADH1,ADC1,YOL086C
RelevantLinks
UniProtKB-P00330
NCBI-12643994
GeneID-2538902

AlcoholDehydrogenaseAntibodyBiotinConjugatedDescription

Background
AlcoholDehydrogenaseispartofthezinc-containingalcoholdehydrogenasefamily.Anti-AlcoholDehydrogenasecatalyzesprimarybranchedalcoholstoconverttotheiraldehydeformsandshowssomereactivitywithsecondaryalcohols.Localizedinthecytoplasm,theAnti-AlcoholDehydrogenaseantibodyisidealforresearchersinterestedinMetabolism,SignalTransduction,andCancerresearch.
Synonyms
rabbitanti-AlcoholDehydrogenaseAntibodybiotinConjugation,biotinconjugatedrabbitant-AlcoholDehydrogenaseAntibody,Alcoholdehydrogenase1,AlcoholdehydrogenaseI,YADH-1,ADH1,ADC1,ADH,ADC
Immunogen
AlcoholDehydrogenase[Yeast]
ImmunogenType
NativeProtein
StorageCondition
Storevialat4°Cpriortorestoration.  Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse. 
ApplicationNote
Anti-AlcoholDehydrogenasehasbeenassayedagainst1.0ugofAlcoholDehydrogenaseinastandardcaptureELISAusingPeroxidaseConjugatedStreptavidin#S000-03andABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:4,000to1:20,000ofthereconstitutionconcentrationissuggestedforthisproduct.
Purity/Specifity
Anti-AlcoholDehydrogenaseisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Biotin,anti-RabbitSerumaswellaspurifiedandpartiallypurifiedAlcoholDehydrogenase[Yeast]. CrossreactivityagainstAlcoholDehydrogenasefromothersourcesmayoccurbuthavenotbeenspecificallydetermined.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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