Rockland/APG7 Antibody/600401487/100 µg

APG7AntibodyProperties

Description

Anti-Apg7(RABBIT)Antibody-600-401-487

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

human

Clonality

Polyclonal

Application

ELISA:1:300,000-1:400,000
IFMicroscopy:UserOptimized
WesternBlot:1:500-1:2,000
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Unconjugated

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

Stabilizer

None

GeneName

ATG7

RelevantLinks

UniProtKB-O95352
NCBI-O95352.1
GeneID-10533

APG7AntibodyDescription

Background

Apg7isthehumangeneproductsimilarinhomologytoPichiapastorisGSA7andSaccharomycescerevisiaeApg7.Intheyeast,theproteinappearstoberequiredforfusionofperoxisomalandvaculuolarmembranes.TheproteinshowshomlogytotheATP-bindingandcatalyticsitesoftheE1ubiquitinactivatingenzymes.

Synonyms

rabbitanti-APG7Antibody,APG-7,APG7,APG7autophagy7-likeantibody,APG7likeantibody,APG7Lantibody,ATG7antibody,ATG-7antibody,ATG7autophagyrelated7homologantibody

Immunogen

Thisaffinitypurifiedantibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingaa558-569ofHumanApg7protein.

ImmunogenType

Peptide

StorageCondition

Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

ThisaffinitypurifiedantibodyhasbeentestedforuseinELISAandbywesternblot. Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandapproximately78kDainsizecorrespondingtohumanApg7bywesternblottingintheappropriatecelllysateorextract. HumanApg7hasbeenreportedtoformahomodimeratapproximately160kDa.

Purity/Specifity

Thisisanaffinitypurifiedantibodyproducedbyimmunoaffinitychromatographyusingtheimmunizingpeptideafterimmobilizationtoasolidphase.ReactivityoccursagainsthumanApg7protein. However,BLASTanalysisindicates100%homology(12/12)forthisproteinfromhuman,chimpanzee,mouse,rat,dog,andchickensources. Thecoreaminoacidsoftheimmunogen,G-D-S-T-R-D-R-Tare100%identical(8/8)inD.melanogaster. ReactivitywithApg7proteinsfromothersourcesisnotknown.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/APG7 Antibody/600401487/100 µg

APG7AntibodyProperties

APG7AntibodyDescription