Rockland/Akt phospho S473 Antibody/200301268/100 µg

AktphosphoS473AntibodyProperties

Description

Anti-AKTpS473(MOUSE)MonoclonalAntibody-200-301-268

Host

Mouse

TargetSpecies

Human

KnownCrossReactivity

human,mouse,rat,monkey

Clonality

Monoclonal17F6.B11IgG1kappa

Application

ELISA:1:20,000
IFMicroscopy:1:500-1:3,000
FlowCytometry:UserOptimized
WesternBlot:1:500-1:3,000
Immunohistochemistry:20µg/mL
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG1

Label

Unconjugated

Concentration

1.0mg/mlbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

Stabilizer

None

GeneName

AKT1

RelevantLinks

UniProtKB-P31749
NCBI-62241011
GeneID-207

AktphosphoS473AntibodyDescription

Background

PhosphoAKTantibodyissuitableforELISA,immunohistochemistry,immunoprecipitationandwesternblotting.AKTphospho473isacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKTpS473(MOUSE)MonoclonalAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.

Synonyms

mouseanti-AKTpS473Antibody,phosphoAKT,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1,AKT1,AKT-1

Immunogen

Anti-AKTpS473(MOUSE)MonoclonalAntibodywasproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoresiduessurroundingS473ofhumanAKT1protein,followedbyhybridomadevelopment.

ImmunogenType

Peptide

PostTranslationalModification

Phosphorylation

StorageCondition

StoreAnti-AKTpS473(MOUSE)MonoclonalAntibodyat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

Expectabandapproximately56kDainsizecorrespondingtophosphorylatedAKTproteinbywesternblottingintheappropriatecelllysateorextract.Thisphospho-specificmonoclonalantibodyreactswithhumanandmouseAKTpS473andshowsminimalreactivitybyELISAagainstthenon-phosphorylatedformoftheimmunizingpeptide.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistryuseformalin-fixedparaffin-embeddedsections.Nopre-treatmentofsampleisrequired.

Purity/Specifity

Anti-AKTpS473MonoclonalAntibodywaspurifiedfromconcentratedtissueculturesupernatebyProteinAchromatography.ThisphosphospecificmonoclonalantibodyisspecificforphosphorylatedhumanandmouseAKTproteinatS473.ABLASTanalysiswasusedtosuggestcross-reactivitywithAKTpS473fromhuman,mouse,ratandchimpanzeesourcesbasedon100%homologywiththeimmunizingsequence.Cross-reactivitywithAKTfromothersourceshasnotbeendetermined.Cross-reactivitywithAKT2andAKT3hasnotbeendetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Akt phospho S473 Antibody/200301268/100 µg

AktphosphoS473AntibodyProperties

AktphosphoS473AntibodyDescription