Rockland/Akt phospho T308 Dylight 488 Conjugated Antibody/200341269/100 µg

AktphosphoT308Dylight488ConjugatedAntibodyProperties

Description

Anti-AKTpT308(MOUSE)MonoclonalAntibodyDyLight™488Conjugated-200-341-269

Host

Mouse

TargetSpecies

Human

KnownCrossReactivity

human,mouse,rat,monkey

Clonality

Monoclonal18F3.H11IgG1kappa

Application

ELISA:1:4,000-1:20,000
FLISA:>1:20,000
IFMicroscopy:>1:5,000
FlowCytometry:UserOptimized
WesternBlot:1:10,000-1:25,000
OtherDilution:UserOptimized

PhysicalState

Lyophilized

ShippingCondition

Ambient

Format

IgG1

Label

DyLight™488

FPValue

3.1molesDyLight™488permoleofIgG1

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

ReconstitutionVolume

100µL

ReconstitutionBuffer

Restorewithdeionizedwater(orequivalent)

Preservative

0.01%(w/v)SodiumAzide

Stabilizer

10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree

GeneName

AKT1

RelevantLinks

UniProtKB-P31749
NCBI-62241011
GeneID-207

AktphosphoT308Dylight488ConjugatedAntibodyDescription

Background

AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.

Synonyms

mouseanti-AKTpT308DyLight™488conjugatedAntibody,DyLight™488conjugatedmouseanti-AKTpT308Antibody,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1,AKT1,AKT-1

Immunogen

ThismonoclonalantibodywasproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoresiduessurroundingT308ofhumanAKT1protein.

ImmunogenType

Peptide

PostTranslationalModification

Phosphorylation

StorageCondition

Storevialat4°Cpriortorestoration. Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.

ApplicationNote

Thismonoclonalantibodyissuitableforimmunofluorescencemicroscopy,FLISAandwesternblotting.Expectabandapproximately56kDainsizecorrespondingtophosphorylatedAKTproteinbywesternblottingintheappropriatecelllysateorextract.Thisphospho-specificmonoclonalantibodyreactswithhumanandmouseAKTpT308andshowsminimalreactivitybyELISAagainstthenon-phosphorylatedformoftheimmunizingpeptide.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.

Purity/Specifity

ThisproductwaspurifiedfromconcentratedtissueculturesupernatebyProteinAchromatography.ThisantibodyisspecificforhumanandmouseAKTproteinphosphorylatedatT308.ABLASTanalysiswasusedtosuggestcross-reactivitywithAKTpT308frommostvertebratespeciessourcesbasedon100%homologywiththeimmunizingsequence.Cross-reactivitywithAKTfromothersourceshasnotbeendetermined.Cross-reactivitywithAKT2andAKT3willlikelyoccur.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Akt phospho T308 Dylight 488 Conjugated Antibody/200341269/100 µg

AktphosphoT308Dylight488ConjugatedAntibodyProperties

AktphosphoT308Dylight488ConjugatedAntibodyDescription