Rockland/Alpha A Crystallin Antibody/200301E84/200 µg

AlphaACrystallinAntibodyProperties

Description

Anti-AlphaACrystallin(MOUSE)MonoclonalAntibody-200-301-E84

Host

Mouse

TargetSpecies

Human

KnownCrossReactivity

Human,Mouse,Bovine,Rat

Clonality

Monoclonal1H3.B8IgG1

Application

ELISA:1:200
WesternBlot:1:2000

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG1

Label

Unconjugated

Concentration

1.0mg/mlbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Stabilizer

50%(v/v)Glycerol

GeneName

CRYAA

RelevantLinks

NCBI-NP_000385.1
GeneID-1409
UniProtKB-P02489

AlphaACrystallinAntibodyDescription

Background

Thealpha-crystallinsaremajorwater-solublelensstructuralproteinsofthevertebrateeyethatarerelatedtothesmallheatshockproteinfamily.Thealpha-crystallinspossessstructuralandfunctionalsimilaritieswithHsp25andHsp27.Mammalianlenscystallinsaredividedintoalpha,betaandgammafamilies.Alphaandbetafamiliesarefurtherdividedintoacidicandbasicgroups(Alpha-AandAlpha-Brespectively).Inthelens,alpha-crystallinprimarilyfunctionstomaintainproperrefractiveindex,howeveritcanalsofunctionasamolecularchaperonethatbindstothedenaturedproteins,keepingtheminsolutionandtherebymaintainingthetranslucencyofthelens.Whencellularstressoccurs,alpha-crystallinentersits’phosphorylatedstateandmayserveastructuralcontrolfunctionandplayaroleinproteinmaintenance.Inadditiontotheirinteractionwithproteins,alpha-crystallinsalsointeractwithnativemoleculessuchasmembraneproteins,Golgimatrixprotein,structuralproteins,nuclearproteinsandDNA.Twootherfunctionsareanautokinaseactivityandparticipationintheintracellulararchitecture,andithasalsobeenproventhatbothalpha-AandBpreventapoptosisbyinhibitingcaspases.

Synonyms

Heatshockproteinbeta4,Acry1,CRYA1,CRYAA,HspB4,Alpha-crystallinAchain

Immunogen

AlphaCrystallinAntibodywasproducedinmicebyrepeatedimmunizationswithNativeAlphaCrystallinprotein.

ImmunogenType

NativeProtein

StorageCondition

Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

Anti-AlphaACrystallinAntibodyisrecommendedforuseinWBandELISA.Expectabandapproximately~20kDaproteincorrespondingtothemolecularmassofa-crystallinonSDSPAGEimmunoblots.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.

Purity/Specifity

Anti-AlphaACrystallinAntibodywaspurifiedbyProteinGchromatography.ABLASTanalysiswasusedtosuggestcross-reactivitywithAlphaACrystallinfromHuman,Mouse,Cow,andRatbasedon100%homologywiththeimmunizingsequence.Doesnotcross-reactwithaB-crystallin,ßL-crystallin,?H-crystallin,?-crystallin,Hsp25,Hsp27orHsp47proteins.Cross-reactivitywithAlphaACrystallinfromothersourceshasnotbeendetermined.Chaperones&Traffickingresearch.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Alpha A Crystallin Antibody/200301E84/200 µg

AlphaACrystallinAntibodyProperties

AlphaACrystallinAntibodyDescription