Rockland/Acinus Antibody/600401X61/100 µg

AcinusAntibodyProperties

Description

Anti-Acinus(RABBIT)Antibody-600-401-X61

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

Human

Clonality

Polyclonal

Application

ELISA:1:10,000
IFMicroscopy:10µg/mL
WesternBlot:1µg/mL
OtherDilution:Immunocytochemistry:10µg/mL

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Unconjugated

Concentration

1mg/mLbyUVabsorbanceat280nm

Buffer

0.01MSodiumPhosphate,0.25MSodiumChloride,pH7.2

Preservative

0.02%(w/v)SodiumAzide

Stabilizer

None

GeneName

ACIN1

RelevantLinks

UniProtKB-Q9UKV3
GeneID-22985
NCBI-AAD56724

AcinusAntibodyDescription

Background

Chromatincondensationandnuclearfragmentation(CCNF)isthehallmarkofapoptosis.CCNFistriggeredbytheactivationofmembersofcaspasefamily,caspaseactivatedDNase(CAD/DFF40),andseveralnovelproteinsincludingAIFandCIDE.AnewinducerofchromatincondensationwasrecentlyidentifiedanddesignatedAcinus(forapoptoticchromatincondensationinducerinthenucleus).Acinusiscleavedbycaspase-3andanadditionalunknownproteasegeneratingasmallactivepeptidep17,whichcauseschromatincondensationinvitrowhenitisaddedtopurifiednuclei.Acinusalsoinducesapoptoticchromatincondensationincells.Acinusisubiquitouslyexpressed.ThreedifferentsplicedformsofAcinushavebeenidentifiedinhumanandmouseanddesignatedAcinusL,AcinusSandAcinusS.

Synonyms

AcinusAntibody,ACN,ACINUS,fSAP152,KIAA0670,Apoptoticchromatincondensationinducerinthenucleus,Acinus

Immunogen

Anti-AcinusantibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithapeptidecorrespondingtoaminoacidsneartheC-terminusofthecleavedactivepeptidep17,whichareidenticaltothoseofmouseAcinus.

ImmunogenType

Peptide

StorageCondition

Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

Anti-AcinusAntibodyhasbeentestedforuseinELISA,Immunocytochemistry,ImmunofluorescenceandWesternBlotting.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandat~220kDainsizecorrespondingtoAcinusproteinbyWesternBlottingintheappropriatecelllysateorextract.

Purity/Specifity

Anti-AcinusAntibodywasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitychromatography.CrossreactivitywithAcinusfromothersourceshasnotbeendetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Acinus Antibody/600401X61/100 µg

AcinusAntibodyProperties

AcinusAntibodyDescription