Rockland/ACSS2 Antibody/600401GW7/100 µg

ACSS2AntibodyProperties

Description

Anti-ACSS2(RABBIT)Antibody-600-401-GW7

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

human

Clonality

Polyclonal

Application

ELISA:1:10,000-1:50,000
WesternBlot:1.0ug/ml

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Unconjugated

Concentration

1.01mg/mlbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

Stabilizer

None

GeneName

ACSS2

Paired

CellViability

RelevantLinks

UniProtKB-Q9NR19
NCBI-NP_001070020.2
GeneID-55902

ACSS2AntibodyDescription

Background

Thisantibodyisdesigned,produced,andvalidatedaspartofacollaborationbetweenRocklandandtheNationalCancerInstitute(NCI).ACSS2(Acyl-CoAsynthetaseshort-chainfamilymember2)isaproteincodinggeneencodingacytosolicenzymethatcatalyzestheactivationofacetateforuseinlipidsynthesisandenergygeneration.Thisproteinactsasamonomerandproducesacetyl-CoAfromacetateinareactionthatrequiresATP.Expressionofthisgeneisregulatedbysterolregulatoryelement-bindingproteins,transcriptionfactorsthatactivategenesrequiredforthesynthesisofcholesterolandunsaturatedfattyacids.Thisproteinisupregulatedinmanycancersandisimportantforpro-survivalunderhypoxicconditions.ACSS2maybeassociatedwithdisorderssuchasCystoisosporiasis.Anti-ACSS2Antibodyisusefulforresearchersinterestedinparasites,intestines,GlucoseEnergyMetabolism,andAKTresearch.

Synonyms

rabbitanti-ACSS2,rabbitantiACSS2,Acetyl-coenzymeAsynthetase(cytoplasmic),Acetate-CoAligase,Acetyl-CoAsynthetase,ACS,AceCS,Acyl-CoAsynthetaseshort-chainfamilymember2,Acyl-activatingenzyme,ACSS2,ACAS2

Immunogen

ACSS2affinitypurifiedantibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithsyntheticpeptideneartheN-termofhumanACSS2.

ImmunogenType

Peptide

StorageCondition

Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

Anti-ACSS2AntibodyhasbeentestedinWesternBlotandELISA.Expectabandatapproximately76kDainwesternblotusingappropriatelysates.PositivecontrolwholecelllysatesusedHep-G2at1µg/mLforWB.Althoughnottested,thisantibodyislikelyfunctionalinimmunohistochemistry,immunofluorescence,andimmunoprecipitation.

Purity/Specifity

ThisaffinitypurifiedantibodyisdirectedagainsthumanACSS2protein.Anti-ACSS2Antibodytowasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitychromatography.Blastanalysisofthesequenceoftheimmunogenshows100%identitywithhumanand80%identitytomouse.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/ACSS2 Antibody/600401GW7/100 µg

ACSS2AntibodyProperties

ACSS2AntibodyDescription