Rockland/Alkaline Phosphatase Antibody Biotin Conjugated/20046340100/100 µg

AlkalinePhosphataseAntibodyBiotinConjugatedProperties

Description

Anti-ALKALINEPHOSPHATASE(E.coli)(RABBIT)AntibodyBiotinConjugated-200-4634-0100

Host

Rabbit

TargetSpecies

Escherichiacoli

KnownCrossReactivity

E.coli

Clonality

Polyclonal

Application

ELISA:1:4,000-1:20,000
WesternBlot:1:500-1:2,000
OtherDilution:UserOptimized

PhysicalState

Lyophilized

ShippingCondition

Ambient

Format

IgG

Label

Biotin

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

ReconstitutionVolume

100µL

ReconstitutionBuffer

Restorewithdeionizedwater(orequivalent)

Preservative

0.01%(w/v)SodiumAzide

Stabilizer

10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree

GeneName

phoA

RelevantLinks

UniProtKB-P00634
NCBI-P00634.1
GeneID-945041

AlkalinePhosphataseAntibodyBiotinConjugatedDescription

Background

AtahighpH,Anti-AlkalinePhosphataseantibodiesstripphosphategroupsfromDNA,RNA,andproteins.ALPismostabundantinplacentalandintestinaltissuespecificsuchastheliver,placenta,andbone.Locatedinthecellmembrane,Anti-AlkalinePhosphataseantibodiesareidealforresearchersinvolvedinCancer,Enzyme,TagsandCellMarker,andCellCycleresearch.

Synonyms

rabbitanti-AlkalinePhosphataseAntibodybiotinConjugation,biotinconjugatedrabbitanti-AlkalinePhosphataseAntibody,Akp2antibody,ALPantibody,Nagaoisozymeantibody,Placentalalkalinephosphataseantibody,Placentallikealkalinephosphataseantibody,PLAP1antibody,PLAPlikeantibody,Reganisozymeantibody,Kasaharaisozymeantibody

Immunogen

AlkalinePhosphatase[E.coli]

ImmunogenType

NativeProtein

StorageCondition

Storevialat4°Cpriortorestoration. Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.

ApplicationNote

Anti-AlkalinePhosphatasehasbeenassayedagainst1.0ugofAlkalinePhosphataseinastandardcaptureELISAusingPeroxidaseConjugatedStreptavidin#S000-03andABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:4,000to1:20,000ofthereconstitutionconcentrationissuggestedforthisproduct.

Purity/Specifity

Anti-AlkalinePhosphataseisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Biotin,anti-RabbitSerumaswellaspurifiedandpartiallypurifiedAlkalinePhosphatase[E.coli]. CrossreactivitiesagainstAlkalinePhosphatasefromothersourcesmayoccurbuthavenotbeenspecificallydetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Alkaline Phosphatase Antibody Biotin Conjugated/20046340100/100 µg

AlkalinePhosphataseAntibodyBiotinConjugatedProperties

AlkalinePhosphataseAntibodyBiotinConjugatedDescription