Rockland/AntiPyruvate Kinase Antibody/2001678S/25 µL

Anti-PyruvateKinaseAntibodyProperties

Description

Anti-PYRUVATEKINASE(RabbitMuscle)(GOAT)AntibodyBiotinConjugated-200-1678S

Host

Goat

TargetSpecies

Rabbit

KnownCrossReactivity

rabbit

Clonality

Polyclonal

Application

WesternBlot:1:200-1:1,000
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Biotin

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

Stabilizer

10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree

GeneName

PKM2

RelevantLinks

UniProtKB-P11974
NCBI-NP_001182574.1
GeneID-100008676

Anti-PyruvateKinaseAntibodyDescription

Background

PyruvateKinaseisanenzymethatiskeyintheglycolyticprocess.PyruvateKinasesspecificactivityistocatalyzethetransferofaphosphategroupfromphosphoenolpyruvatetoADP,yieldingonemoleculeofpyruvateandonemoleculeofATP.AisoenzymeofPyruvateKinase,knownastheM2,isakeyareaofstudyintumorigenesis,astheM2isoenzymeseemstoelevateaerobicglycolysisintumorcells,leadingtotumorproliferation.Conflictingevidenceastotheimportanceofthisisoenzymeintumorgrowthestablishesthisasalargeareaofexplporationandstudy.Anti-PyurvateKinaseAntibodyisidealforinvestigatorsinCancer,CellBiology,andSignalTransductionresearch.

Synonyms

goatanti-PyruvateKinaseAntibody,biotinConjugatedgoatanti-PyruvateKinaseAntibody,PyruvatekinaseisozymesM1/M2antibody,Pyruvatekinasemuscleisozymeantibody,Pyruvatekinase2/3antibody,Cytosolicthyroidhormone-bindingproteinantibody

Immunogen

PyruvateKinase[RabbitMuscle]

ImmunogenType

NativeProtein

StorageCondition

Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.

ApplicationNote

Anti-PyruvateKinaseAntibodyhasbeenassayedagainst1.0ugofPyruvateKinaseinastandardcaptureELISAusingPeroxidaseConjugatedStreptavidin#S000-03andABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:3,000to1:15,000ofthereconstitutionconcentrationissuggestedforAnti-PyruvateKinaseAntibody.

Purity/Specifity

Anti-PyruvateKinaseAntibodyisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Biotin,anti-GoatSerumaswellaspurifiedandpartiallypurifiedPyruvateKinase[RabbitMuscle].Anti-PyruvateKinasehasbeenreportedtoreactwithallformsofpyruvatekinase(panM-PK). CrossreactivityagainstPyruvateKinasefromothersourcesmayoccurbuthavenotbeenspecificallydetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/AntiPyruvate Kinase Antibody/2001678S/25 µL

Anti-PyruvateKinaseAntibodyProperties

Anti-PyruvateKinaseAntibodyDescription