Rockland/Alpha2Macroglobulin Antibody/2001012070100/100 µg

Alpha-2-MacroglobulinAntibodyProperties

Description

Anti-alpha-2-MACROGLOBULIN(GOAT)Antibody-200-101-207-0100

Host

Goat

TargetSpecies

Human

KnownCrossReactivity

human

Clonality

Polyclonal

Application

ELISA:1:80,000-1:400,000
WesternBlot:1:2,000-1:20,000
Immunohistochemistry:UserOptimized
OtherDilution:UserOptimized

SpeciesofOrigin

goat

PhysicalState

Lyophilized

ShippingCondition

Ambient

Format

IgG

Label

Unconjugated

Concentration

1.0mg/mlbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

ReconstitutionVolume

100µL

ReconstitutionBuffer

Restorewithdeionizedwater(orequivalent)

Preservative

0.01%(w/v)SodiumAzide

Stabilizer

None

GeneName

A2M

RelevantLinks

UniProtKB-P01023
NCBI-P01023.3
GeneID-2

Alpha-2-MacroglobulinAntibodyDescription

Background

Alpha-2-MacroglobulindetectsMacroglobulin.Alpha-2-MACROGLOBULINisalargeplasmaproteinfoundintheblood.Itisproducedbytheliver,andisamajorcomponentofthealpha-2bandinproteinelectrophoresis.Alpha2-Macroglobulinisthelargestmajornonimmunoglobulinproteininplasma.Thealpha2-macroglobulinmoleculeissynthesizedmainlyinliver,butalsolocallybymacrophages,fibroblasts,andadrenocorticalcells.Alpha2macroglobulinactsasanantiproteaseandisabletoinactivateanenormousvarietyofproteinases.Itfunctionsasaninhibitoroffibrinolysisbyinhibitingplasminandkallikrein.Itfunctionsasaninhibitorofcoagulationbyinhibitingthrombin.Alpha2-macroglobulinmayactasacarrierproteinbecauseitalsobindstonumerousgrowthfactorsandcytokines,suchasplatelet-derivedgrowthfactor,basicfibroblastgrowthfactor,TGF-ß,insulin,andIL-1ß.Nospecificdeficiencywithassociateddiseasehasbeenrecognized,andnodiseasestateisattributedtolowconcentrationsofAlpha2macroglobulin.Theconcentrationofalpha2macroglobulinrises10-foldormoreinthenephroticsyndromewhenotherlowermolecularweightproteinsarelostintheurine.Thelossofalpha2macroglobulinintourineispreventedbyitslargesize.Thenetresultisthatalpha2macroglobulinreachesserumlevelsequaltoorgreaterthanthoseofalbumininthenephroticsyndrome,whichhastheeffectofmaintainingoncoticpressure.Antialpha-2-macroglobulinisidealforinvestigatorsinvolvedinserumcomponentproteinresearch.

Synonyms

goatanti-Alpha-2-MacroglobulinAntibody,A2Mantibody,Alpha2Mantibody,Alpha2Mantibody,CPAMD5antibody,DKFZp779B086antibody,FWP007antibody,S8637antibody

Immunogen

a2-Macroglobulin[HumanPlasma]

ImmunogenType

NativeProtein

StorageCondition

Storevialat4°Cpriortorestoration.Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

Anti-alpha-2-macroglobulinantibodyissuitableforELISA,WesternBlotandImmunohistochemistryapplications.Anti-alpha-2-MACROGLOBULINantibodyshouldbeoptimizedbytheenduserforthespecificreactiveconditions.

Purity/Specifity

Anti-alpha-2-MACROGLOBULINisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-GoatSerumaswellaspurifiedandpartiallypurifieda2-Macroglobulin[HumanPlasma]. Crossreactivityagainsta2-Macroglobulinfromothersourcesisunknown.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Alpha2Macroglobulin Antibody/2001012070100/100 µg

Alpha-2-MacroglobulinAntibodyProperties

Alpha-2-MacroglobulinAntibodyDescription