Rockland/AKT1 Western Chemiluminescent Blotting Kit/KCAI51/1 Kit

AKT1WesternChemiluminescentBlottingKitProperties

Description

AKT1WesternBlotKit-KCAI51

Host

Mouse

TargetSpecies

Human

KnownCrossReactivity

human

Clonality

Monoclonal14E5.A2.B2.H9IgG2akappa

Application

WesternBlot:UserOptimized
OtherDilution:UserOptimized

ProductType

Kit

KitType

ChemiluminescentWesternBlotKit

PhysicalState

n/a

ShippingCondition

DryIce

Format

IgG2a

Label

Unconjugated

Preservative

WashbuffersMUSTNOTcontainSODIUMAZIDEorotherinhibitorsofperoxidaseactivity!

Stabilizer

None

GeneName

AKT1

RelevantLinks

UniProtKB-P31749
AKTChemiluminescentWBlotKitProtocol

AKT1WesternChemiluminescentBlottingKitDescription

Background

AKT1WesternBlotingkitallowsforthedetectionofendogenousproteinlevelsofhumanAKT1presentincelllysatesprovidedbytheuser.Asapositivecontrol,thiskitincludesaMDA-MB468wholecelllysateproventocontainAKT1.AfterproteinseparationbySDS-PAGEandtransfer,themembraneisprobedwithRocklandsoptimizedAnti-AKT1antibody.Detectionofthemembraneboundantibody-antigencomplexisachievedbytheadditionofasecondaryantibodyconjugatedtotheenzymehorseradishperoxidase.Theenzymereactswithaspecializedformulationofluminol,anextremelysensitive,non-radioactivesubstratethatemitslightandallowsvisualizationusingX-rayfilmorotherimagingmethods,includinghighlysensitiveCCDcamerasandimagingsystems.AKT1WesternBlotingisidealforinvestigatorsinvolvedinCellSignaling,Cancer,NeuroscienceandSignalTransductionresearch.

Synonyms

AKT1westernblotting,AKT-1,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt1antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gamma,ChemiluminescentWB,WesternBlottingKit,ChemiluminescentKit,PeroxidaseKit,ImmunoblottingKit

StorageCondition

Seekitinsertforcompleteinstructions.

ApplicationNote

UseRocklandImmunochemicals’Anti-AKT1ChemiluminescentKitforWesternBlottingfordetectionofhumanAKT1nativeandrecombinantproteinsbywesternblot.Expectabandapproximately56kDainsizecorrespondingtoAKT1protein.Thiskitisusefulforbothwesternblottinganddotblottingmethods.Pleasereadtheentireproductinsertpriortouse.

Purity/Specifity

RocklandImmunochemicals’ChemiluminescentWesternBlotKitforAKT1combinesallofthenecessaryreagentswitharapidprovenprotocolandtheextremelyhighsignaldetectionofourluminolchemiluminescentsubstrateforthedetectionofnativeand/orrecombinanthumanAKT1protein.Thekitisdesignedtodetectbothunphosphorylatedandphosphorylatedformsoftheprotein.CrossreactivitywithAKT1fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows100%identitytohuman,mouse,andrat,therefore,crossreactivityisexpected.Cross-reactivitywithAKT2andAKT3hasnotbeendetermined.Thiskitcontainssufficientsubstrateforupto5miniblotsat7.5x8cm2(1,800cm2)andisstableforatleast1yearwhenstoredasindicated.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Rockland/AKT1 Western Chemiluminescent Blotting Kit/KCAI51/1 Kit

AKT1WesternChemiluminescentBlottingKitProperties

AKT1WesternChemiluminescentBlottingKitDescription