Rockland/Alkaline Phosphatase Antibody Peroxidase Conjugated/2004031420100/100 µg

AlkalinePhosphataseAntibodyPeroxidaseConjugatedProperties

Description

Anti-ALKALINEPHOSPHATASE(HumanIntestine)(RABBIT)AntibodyPeroxidaseConjugated-200-403-142-0100

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

human

Clonality

Polyclonal

Application

WesternBlot:1:500-1:2,000
OtherDilution:UserOptimized

PhysicalState

Lyophilized

ShippingCondition

Ambient

Format

IgG

Label

Peroxidase(Horseradish)

Concentration

1mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

ReconstitutionVolume

100µL

ReconstitutionBuffer

Restorewithdeionizedwater(orequivalent)

Preservative

0.01%(w/v)GentamicinSulfate.DoNOTaddSodiumAzide!

Stabilizer

10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree

GeneName

ALP1

RelevantLinks

UniProtKB-P09923
NCBI-P09923.2
GeneID-248

AlkalinePhosphataseAntibodyPeroxidaseConjugatedDescription

Background

Alkalinephosphatase(ALP,ALKP)isahydrolaseenzymeresponsibleforremovingphosphategroupsfrommanytypesofmolecules,includingnucleotides,proteins,andalkaloids.Theprocessofremovingthephosphategroupiscalleddephosphorylation.Allmammalianalkalinephosphataseisoenzymesexceptplacental(PALPandSEAP)areinhibitedbyhomoarginine,and,insimilarmanner,allexcepttheintestinalandplacentalonesareblockedbylevamisole.Heatingfor~2hoursat65°CinactivatedmostisoenzymesexceptPlacentalisoforms(PALPandSEAP).

Synonyms

rabbitanti-AlkalinePhosphataseAntibodyPeroxidaseConjugation,HRPconjugatedrabbitanti-Alkalinephosphataseintestinalantibody,Alkalinephosphomonoesteraseantibody,ALPIantibody,Glycerophosphataseantibody,IAPantibody,Intestinalalkalinephosphataseantibody,Kasaharaisozymeantibody

Immunogen

AlkalinePhosphatase[HumanIntestine]

ImmunogenType

NativeProtein

StorageCondition

Storevialat4°Cpriortorestoration. Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.

ApplicationNote

Anti-AlkalinePhosphatasehasbeenassayedagainst1.0ugofAlkalinePhosphatase[HumanIntestine]byimmunoblotusingTMB3,3,5,5Tetramethylbenzidinecode#TMBM-100asasubstrateatroomtemperature. Aworkingdilutionof1:1,000to1:5,000ofthereconstitutionconcentrationissuggestedforthisproduct.

Purity/Specifity

AlkalinePhosphataseisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-Peroxidase,anti-RabbitSerumaswellaspurifiedandpartiallypurifiedAlkalinePhosphatase[HumanIntestine]. CrossreactivityagainstAlkalinePhosphatasefromothersourcesmayoccurbuthasnotbeenspecificallydetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Alkaline Phosphatase Antibody Peroxidase Conjugated/2004031420100/100 µg

AlkalinePhosphataseAntibodyPeroxidaseConjugatedProperties

AlkalinePhosphataseAntibodyPeroxidaseConjugatedDescription