Rockland/AKT Antibody/200401N98/100 µg

AKTAntibodyProperties

Description

Anti-AKT(RABBIT)Antibody-200-401-N98

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

Human,Mouse,Rat

Clonality

Polyclonal

Application

ELISA:UserOptimized
IFMicroscopy:1:500-1:2,000
FlowCytometry:UserOptimized
WesternBlot:1:1,000-1:5,000
Immunohistochemistry:1:500-1:2,000
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Unconjugated

Concentration

1.0mg/mlbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.5MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

Stabilizer

None

GeneName

AKT1,AKT2,AKT3

RelevantLinks

UniProtKB-P31749
NCBI-NP_001014431.1
GeneID-207
UniProtKB-Q9Y243
NCBI-NP_001193658.1
GeneID-10000
UniProtKB-P31751
NCBI-NP_001617.1
GeneID-208

AKTAntibodyDescription

Background

AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKTAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.

Synonyms

rabbitanti-AKTantibody,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1Antibody,AKT2Antibody,AKT3Antibody

Immunogen

Anti-AKTPanReactiveAntibodywasproducedinrabbitsbyrepeatedimmunizationswithacocktailcontainingrecombinanthumanAKT1,AKT2,andAKT3proteinisoforms.

ImmunogenType

NativeProtein

StorageCondition

Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

AKTAntibodyissuitableforwesternblottingtodetectasinglebandoftheexpectedapparentmolecularweight,immunohistochemistry(Formalin-fixedparaffin-embeddedsections),immunofluorescencemicroscopyusingparaformaldehyde-fixedprimarycardiomyocytecultures,immunoprecip-itation,transfectedcellculture,primarycellcultureandELISA.Expectbandsat~56kDainsizecorrespondingtoAKTbywesternblottingintheappropriatecelllysateorextract.Researchersshoulddetermineoptimaltitersforapplicationsthatarenotstatedbelow.

Purity/Specifity

AKTAntibodyreactswiththeAKTfromhumantissues.Anti-AKTantibodywaspurifiedfrommonospecificantiserumbyProteinAchromatography.Basedonsequenceweexpectthisantibodytoreactaswellwithrat,mouse,andchickenAKT.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/AKT Antibody/200401N98/100 µg

AKTAntibodyProperties

AKTAntibodyDescription