Rockland/Ambra1 Antibody/600401Y08/100 µg

Ambra1AntibodyProperties

Description

Anti-Ambra1(RABBIT)Antibody-600-401-Y08

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

Human,mouse,rat

Clonality

Polyclonal

Application

ELISA:1:10,000
IFMicroscopy:20µg/mL
WesternBlot:1µg/mL
Immunohistochemistry:5µg/mL

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Unconjugated

Concentration

1mg/mLbyUVabsorbanceat280nm

Buffer

0.01MSodiumPhosphate,0.25MSodiumChloride,pH7.2

Preservative

0.02%(w/v)SodiumAzide

Stabilizer

None

GeneName

AMBRA1

RelevantLinks

UniProtKB-Q9C0C7
GeneID-55626
NCBI-Q9C0C7

Ambra1AntibodyDescription

Background

Autophagy,theprocessofbulkdegradationofcellularproteinsthroughanautophagosomic-lysosomalpathwayisimportantfornormalgrowthcontrolandmaybedefectiveintumorcells.Itisinvolvedinthepreservationofcellularnutrientsunderstarvationconditionsaswellasthenormalturnoverofcytosoliccomponents.Beclin-1,aprincipalregulatorofautophagosomeformation,isinturnregulatedbyAmbra1.Ambra1associateswithBeclin-1througharegionnearitscenterasdeterminedbyyeasttwo-hybridassay.Nullmutationsinthisgeneinmiceresultedinembryoniclethalitywithsevereneuraltubedefectsassociatedwithautophagyimpairment,accumulationofubiquitinatedproteins,unbalancedcellproliferationandexcessiveapoptoticdeath.Furthermore,down-regulationofAmbra1inculturedcellsthoughRNAinterferencedecreasedthelevelofrapamycin-andnutrientstarvation-inducedautophagy.MultipleisoformsofAmbra1areknowntoexist.

Synonyms

Ambra1Antibody,DCAF3,WDR94,KIAA1736,ActivatingmoleculeinBECN1-regulatedautophagyprotein1

Immunogen

Anti-Ambra1antibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswitha15aminoacidsyntheticpeptidefromneartheC-terminusofhumanAmbra1.

ImmunogenType

Peptide

StorageCondition

Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.

ApplicationNote

Anti-Ambra1AntibodyhasbeentestedforuseinELISA,WesternBlotting,ImmunohistochemistryandImmunofluorescence.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandatapproximately143kDainWesternBlotsofspecificcelllysatesandtissues.

Purity/Specifity

Anti-Ambra1Antibodywasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitychromatography.CrossreactivitywithAmbra1fromothersourceshasnotbeendetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Ambra1 Antibody/600401Y08/100 µg

Ambra1AntibodyProperties

Ambra1AntibodyDescription