Rockland/Akt phospho S473 Dylight 649 Conjugated /200343268/100 µg

AktphosphoS473Dylight649ConjugatedProperties

Description

Anti-AKTpS473(MOUSE)MonoclonalAntibodyDyLight™649Conjugated-200-343-268

Host

Mouse

TargetSpecies

Human

KnownCrossReactivity

human,mouse,rat,monkey

Clonality

Monoclonal17F6.B11IgG1kappa

Application

ELISA:1:4,000-1:20,000
FLISA:>1:20,000
IFMicroscopy:>1:5,000
FlowCytometry:UserOptimized
WesternBlot:1:10,000-1:25,000
OtherDilution:UserOptimized

PhysicalState

Lyophilized

ShippingCondition

Ambient

Format

IgG1

Label

DyLight™649

FPValue

1.5molesDyLight™649permoleofIgG1

Concentration

1.0mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

ReconstitutionVolume

100µL

ReconstitutionBuffer

Restorewithdeionizedwater(orequivalent)

Preservative

0.01%(w/v)SodiumAzide

Stabilizer

10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree

GeneName

AKT1

RelevantLinks

UniProtKB-P31749
NCBI-62241011
GeneID-207

AktphosphoS473Dylight649ConjugatedDescription

Background

AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.

Synonyms

mouseanti-AKTpS473DyLight™649conjugatedAntibody,DyLight649conjugatedmouseanti-AKTpS473Antibody,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1,AKT1,AKT-1

Immunogen

ThismonoclonalantibodywasproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoresiduessurroundingS473ofhumanAKT1protein.

ImmunogenType

Peptide

PostTranslationalModification

Phosphorylation

StorageCondition

Storevialat4°Cpriortorestoration. Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing. Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature. Thisproductisstableforseveralweeksat4°Casanundilutedliquid. Diluteonlypriortoimmediateuse.

ApplicationNote

Thismonoclonalantibodyissuitableforimmunofluorescencemicroscopy,FLISAandwesternblotting.Expectabandapproximately56kDainsizecorrespondingtophosphorylatedAKTproteinbywesternblottingintheappropriatecelllysateorextract.Thisphospho-specificmonoclonalantibodyreactswithhumanandmouseAKTpS473andshowsminimalreactivitybyELISAagainstthenon-phosphorylatedformoftheimmunizingpeptide.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.

Purity/Specifity

ThisproductwaspurifiedfromconcentratedtissueculturesupernatebyProteinAchromatography.ThisantibodyisspecificforhumanandmouseAKTproteinphosphorylatedatS473.ABLASTanalysiswasusedtosuggestcross-reactivitywithAKTpS473fromhuman,mouse,ratandchimpanzeesourcesbasedon100%homologywiththeimmunizingsequence.Cross-reactivitywithAKTfromothersourceshasnotbeendetermined.Cross-reactivitywithAKT2andAKT3hasnotbeendetermined.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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Rockland/Akt phospho S473 Dylight 649 Conjugated /200343268/100 µg

AktphosphoS473Dylight649ConjugatedProperties

AktphosphoS473Dylight649ConjugatedDescription