产品资料

ProteinTech/AKT Polyclonal antibody/150ul/10176-2-AP

Tested Applications

Positive WB detected in	A549 cells, HepG2 cells,MCF-7 cells,HeLa cells,HEK-293 cells,NIH/3T3 cells,C6 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue, human cervical cancer tissue,human breast cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in	HeLa cells
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 6 publications below
WB	See 346 publications below
IHC	See 9 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

10176-2-AP targets AKT inWB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	A. sphaerocephala Krasch, blunt snout bream, carp, chicken, cow, fish, hamster, human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AKT fusion protein Ag0213
Full Name	v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight	56 kDa
Observed molecular weight	56-62 kDa
GenBank accession number	BC000479
Gene symbol	AKT1
Gene ID (NCBI)	207
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Protocols

Product Specific Protocols
WB protocol for AKT antibody 10176-2-AP	Download protocol
IHC protocol for AKT antibody 10176-2-AP	Download protocol
IF protocol for AKT antibody 10176-2-AP	Download protocol
IP protocol for AKT antibody 10176-2-AP	Download protocol
FC protocol for AKT antibody 10176-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols