产品资料

ProteinTech/NPTX2 Polyclonal antibody/150ul/10889-1-AP

Tested Applications

Positive WB detected in	mouse brain tissue,PC-3 cells,SH-SY5Y cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human brain tissue,human pancreas cancer tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 2 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

10889-1-AP targets NPTX2 inWB, IP, IHC, IF, ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NPTX2 fusion protein Ag1326
Full Name	neuronal pentraxin II
Calculated molecular weight	47 kDa
Observed molecular weight	47 kDa
GenBank accession number	BC009924
Gene symbol	NPTX2
Gene ID (NCBI)	4885
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for-20^oC storage.

Background Information

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is secreted protein involved in excitatory synapse formation. It also plays a role in clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in the Parkinson"s disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

Protocols

Product Specific Protocols
WB protocol for NPTX2 antibody 10889-1-AP	Download protocol
IHC protocol for NPTX2 antibody 10889-1-AP	Download protocol
IP protocol for NPTX2 antibody 10889-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols