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ThisuniversalmousemonoclonalantibodywasgeneratedagainstplantactinACT1andrecognizesallplant(arABIdopsisACT1,2,3,4,7,8,11,12),animal(e.g.,humanmuscleandcytoplasmic),fungal,andprotistactins.
ActinexistsasaubiquitousproteininvolvedwithfilamentformationthatmakeuplargeportionsoftheCytoskeleton.Actinfilamentsinteractwithmyosintoassistinmusclecontractionaswellasaidingincellmotilityandcytokinesis.Invertebratestherearethreegroupsofactinisoforms:alpha,betaandgamma.Thealphaactinsarefoundinmuscletissuesandareamajorconstituentofthecontractileapparatus.Thebetaandgammaactinsco-existsinmostcelltypesascomponentsofthecytoskeletonandasmediatorsofinternalcellmotility.
FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.
PartofTheInvestigator"sAnnexeprogram.
| ProductType: | Antibody |
| AccessionID: | AEC09427 |
| Antigen: | ArabidopsisActin(ACT1) |
| MolecularWeight: | 45kDa |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| CloneName: | mAbGEa(5-16H1C8) |
| Reactivity: | Human,Mouse,Rat,Bovine,Drosophila,Ovine,Plant,Xenopuslaevis,Yeast,Zebrafish |
| Immunogen: | PurifiedArabidopsisactin(ACT1) |
| SpeciesImmunized: | Mouse |
| PurificationMethod: | ProteinGcolumn |
| Buffer: | 0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide |
| TestedApplications: | WB,IF,IHC,ELISA |
| Storage: | -20C |
| Shipped: | Coldpacks |
| Antibody | Reactivity |
| Actin[mAbGEa] | Universal |
| Actin-1[mAb45a] | ACT1,3,4,12 |
| Actin-11[mAb2345a] | ACT1,3,4,7,11,12 |
| Actin-2[mAb13a] | ACT2,8,11 |
| Actin-8[mAbGPa] | ACT1,2,3,4,7,8,11,12 |
WesternBlotAnalysis
TOP:ReactivitymAbGEawithhuman(HsBandHsA1,native;HsG1andHsA2,recombinant)andplant(At-ACT2,recombinant)actins.ThenumbersontheCoomassiebluestainedgelindicatetheamountofactinproteinloaded.BOTTOM:ReactivityofmAbGEawithC.intestinaliscytoplasmic-likeactinACTB1(CiB)anddiverseprotistactins.Ac,A.castellanii;Mb,
Adaptedfrom:Kandasamy,M.K.,etal.(2012)PlantCell24,2041-57.
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