产品资料

Kerafast/Anti-Actin-7 Antibodies/EGA245/100ug

ThesemousemonoclonalantibodiesweregeneratedagainstArABIdopsisthalianaActin-7andrecognizeA.thalianaActin-7.

Highlights:

ReactwithA.thalianaActin-7(Maydisplayreactivitytowardmultipleotherespecies)
SuitableforWesternBlot,ELISAandImmunofluorescenceapplications

Actin-7hasbeenidentifiedinanimals,plantsandprotists.Duringplantgrowthanddevelopment,thephytohormoneauxininducesawidearrayofchangesthatincludecelldivision,cellexpansion,celldifferentiation,andorganinitiation.IthasbeensuggestedthattheactinCytoskeletonplaysanactiveroleintheelaborationoftheseresponsesbydirectingspecificchangesincellmorphologyandcytoarchitecture.ThepromoterandproteinproductofACT7,anAraBDopsisvegetativeactingene,arerapidlyandstronglyinducedinresponsetoexogenousauxin.ACT7isrequiredfortheformationofcallustissue,andisalsoessentialforgerminationandrootgrowthACT7actingeneisalsoexpressedinrapidlydevelopingtissuesandrespondstoexternalstimuli,suchasexposuretohormones.

FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.

Specifications

ProductType:	Antibody
Name:	Actin-7Antibodies
AccessionID:	P53492
Antigen:	Actin-7
Isotype:	IgG
Clonality:	Monoclonal
CloneName:	29G12.G5.G6,33E8.C11.F5.D1,36H8.C12.H10.B6
Reactivity:	TestedinA.thaliana.Maydisplayreactivitytowardmultipleotherespecies(ND)
Immunogen:	A.thalianaActin-7
SpeciesImmunized:	BALB/cmouse
PurificationMethod:	ProteinG
Buffer:	0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide
TestedApplications:	WB,ELISA,IF(ItisadvisabletouseallthreeMAbsinthefirst-time,qualitativeexperimentalsetuptodeterminewhichisthemostsuitableforaspecificexperiment.ThesemaybeusedyocomplementexcperimentscarriedoutinvolvingmAbGEauniversalanti-actinsantibody.)
Storage:	-20C
Shipped:	Coldpacks

Provider

FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.

Comments

ItisadvisabletouseallthreeMAbsinthefirst-time,qualitativeexperimentalsetuptodeterminewhichisthemostsuitableforaspecificexperiment.ThesemaybeusedyocomplementexcperimentscarriedoutinvolvingmAbGEauniversalanti-actinsantibody.

References

Kandasamy,M.K.,Gilliland,L.U.,McKinney,E.C.,andMeagher,R.B.(2001).Oneplantactinisovariant,ACT7,isinducedbyauxinandrequiredfornormalcallusformation.PlantCell13,1541-54.
Kandasamy,M.K.,McKinney,E.C.,andMeagher,R.B.(1999).Thelatepollen-specificactinsinangiosperms.PlantJ18,681-91.
Kandasamy,M.K.,McKinney,E.C.,Roy,E.,andMeagher,R.B.(2012).Plantvegetativeandanimalcytoplasmicactinssharefunctionalcompetenceforspatialdevelopmentwithprotists.PlantCell24,2041-57.
L.U.Gilliland,L.C.Pawloski,M.K.Kandasamy,andR.B.Meagher,ArabidopsisactingeneACT7playsanessentialroleingerminationandrootgrowth,PlantJournal,vol.33,no.2,pp.319328,2003.
McDowell,JM,An,Y-q,Huang,S,McKinney,EC,MeagherRB:TheArabidopsisACT7ActinGeneIsExpressedinRapidlyDevelopingTissuesandRespondstoSeveralExternalStimuli.PlantPhysiol.(1996)111:699-711.

Ifyoupublishresearchwiththisproduct,pleaseletusknowsowecanciteyourpaper.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Kerafast/Anti-Actin-7 Antibodies/EGA245/100ug