Actin-ToolkitFluorescence Microscopy
Rabbit Skeletal Muscle ATTO594-ActinCat. # 8087-01
Applications
- Microscopical analysis of actin filament bundles or networks;
Description
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Protein | ATTO594-Actin (α-Actin) |
Origin | Skeletal muscle alpha actin, rabbit |
Molecular mass | 42kDa |
Toolkit description | TheActin-ToolkitFluorescence Microscopy was developed to analyze and demonstrate the effect of ligands on actin polymerization, or ligand-induced formation of actin bundles and networks. In the assays,ATTO594-Actin (λex594nm, λem627nm)is mixed with the ligand to observe the effects on either G- or F-actin. In contrast to staining with phalloidin conjugates, which can interfere with ligand binding sites on the actin molecule, no such observations were made with ATTO labeled actin. ATTO-Actins are validated to possess the polymerization properties of native actin. The Toolkit further contains unlabeled actin (0.5mg α-skeletal muscle actin). A mixture of both excludes possible interference of the fluorescent actin conjugates on ligand binding sites.Vinculin tail - a V8 protease cleavage product of vinculin -is an efficientactin bundling vinculin domain, is supplied with the Toolkitas a bundling reference. Several standardized experiments are described step-by-step in the handbook supplied with the kit. In combination with the actinbufferscontained in thekit, error-free handling is guaranteed. The kit contains 4x100µg of α-skeletal muscle ATTO594-Actin conjugates and buffers to keep the actin functional. |
Purity | >99%by scanning densitometry. |
Properties
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Form | Lyophilized, ready-to-use. |
Kit content | 4x100µg ATTO594-Actin (lyophilized powder);1x0.5mg α-skeletal muscle actin (lyophilized powder);1x50µg Vinculin tail;5x50ml MonoMix (lyophilized G-actin ATP-buffer);1x1.0ml PolyMix 10x stock (lyophilized F-actin ATP-buffer, pH 7.4);1x0.5ml 1M MgCl2;1x Toolkit Handbook Fluorescence Microscopy;10x Light protective tubes & tags; |
Actin Buffer | 2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides,when reconstituted with 1.0 mlultrapure water to obtain a 1mg/ml solution. |
Related products | Actin Binding Proteins |
Purification notes | Purified from rabbit skeletal muscle, GPC. |
Protein concentration | Determined by the Biuret method. |
Storage instructions | We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival.Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. | 51005-14-2 (Actin) |
Further Information
Material and Safety Data SheetTable of Dyes and Light Sources
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