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Ezrin(Actin Binding Protein)Cat. #: 8462-02................................................................................................................................................................................Description
Protein | Ezrin (synonym: villin-2) |
Origin | human, recombinant (untagged) |
Molecular mass | 70kDa |
Protein description | Ezrin is probably participating in connecting the cytoskeleton to the plasma membrane. Like many proteins, Ezrin is autoinhibited by a head-tail interaction. |
Actin interaction | The C-ter binds to actin, PIP2 and membrane proteins, e.g. CD44. |
Properties
Form | Lyophilized, ready-to-use. |
Quantity per unit | 1x250 µg |
Buffer | 100mM KCl, 20mM Hepes pH 7.4, 0.2mM DTT and 5% sucrose, when reconstituted with 250 µl ultrapure water to obtain a 1.0 mg/ml solution. |
Purity | >96%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels. |
Purification notes | Ezrin was expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography. |
Protein concentration | Determinedby OD280 (0.1%=0.963) |
Storage instructions | Ezrin isstored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilizedproteinis kept on ice and should be stored at -20°C in glycerol orCryoProtect(Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
ReferencesDisorazolesBlockGroupAStreptococcalInvasionintoEpithelialCellsViaInterferencewith theHostFactorEzrin.
Rox K, Rohde M, Chhatwal GS, Müller R.
Cell Chem Biol. 2017 Feb 16;24(2):159-170. doi: 10.1016/j.chembiol.2016.12.011. Epub 2017 Jan 12.
Solutionstructureofhumancofilin:actinbinding,pHsensitivity, andrelationshiptoactin-depolymerizingfactor.
Pope BJ, Zierler-Gould KM, Kühne R, Weeds AG, Ball LJ.
J Biol Chem. 2004 Feb 6;279(6):4840-8. Epub 2003 Nov 18.
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