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NEM-Myosin II(Actin Binding Protein)Cat. #: 8316-01
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ApplicationTethering protein for TIRF microscopy, biochemical assaysDescription
Protein | NEM-Myosin |
Origin | Skeletal muscle, rabbit |
Molecular mass | ~480kDa |
Protein description | NEM-Myosin II is chemically inactivated fast muscle myosin II from rabbit psoas muscle. Myosin II is a hexameric, actin-binding ATPase with a total molecular weight of 480kD in a head- and-tail conformation.Myosin II has two identical head domains, where the actin binding sites are localized. It consists of two heavy chains (each 220kD) and four light chains (ELC=essential and RLC= regulatory light chains). |
Actin interaction | Binds to F-actin. |
Properties
Form | Lyophilized, ready-to-use. |
Quantity | 1x200 µg |
Buffer | 500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides, when reconstituted with 200 µlultrapure water to obtain a 1mg/ml solution. |
Purity | >98%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels. |
Purification notes | Purified from rabbit skeletal muscle (m. psoas). |
Protein concentration | Determinedat OD280 (0.1% = 0.53). |
Storage instructions | Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
Further Information
Product DataSheet
Material and Safety Data Sheet
References
TAGLN2polymerizesG-actinin alowionicstatebutblocksArp2/3-nucleatedactinbranchinginphysiologicalconditions.
Kim HR, Kwon MS, Lee S, Mun Y, Lee KS, Kim CH, Na BR, Kim BNR, Piragyte I, Lee HS, Jun Y, Jin MS, Hyun YM, Jung HS, Mun JY, Jun CD.
Sci Rep. 2018 Apr 3;8(1):5503. doi: 10.1038/s41598-018-23816-2.
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