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hypermol/Anti-Mouse IgG F(ab)2 ATTO647N (H+L)/2418-0.5MG

Description

Fluorescent Antibody Kit

Anti-Mouse IgG F(ab')2 ATTO647NAntibody produced in goatCat. #: 2418-0.5MG

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Description

Product name

Anti-Mouse IgGF(ab')2 ATTO647N

Target species

Mouse

Description

ATTO-labeled F(ab')2fragments belong to the next generation of fluorescent antibodies, characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgGF(ab')2ATTO647N is specifically suited for high resolution micoscopy, due to the high photostabilty and strong fluorescence quantum yield.

Conjugates of F(ab')2 fragments are preferable to whole antibody conjugates, sincethey do not interact with Fc receptor-bearing membranes, due tothe absence of the antibody Fc region.

Specification

Goat anti-mouse IgG F(ab')2 ATTO647N (H+L), Immunofluorescence: 1:300-1:1000,Degree of labeling (DOL) : >2-5, Unconjugated dye ≤5% of total fluorescence.For further information please see DataSheet.

Optical properties

λex645nm, λem 669nm

Properties

Form

Lyophilized.

Nominal concentration

2 mg/ml

Content

Buffer

0.5mg Anti-Mouse IgG F(ab')2 ATTO647N1 ml Glycerol bufferProduct documentation50% glycerol, 0.01 M sodium phosphate, 0.1 Msodium chloride, 5 mM sodium azide, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Affinity purified IgG, SEC purified after pepsin cleavage.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions

Shipped at ambient temperature.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

ProductDataSheet
Material and Safety Data SheetTable of Dyes and Light SourcesCatalogue: ATTO Secondary Antibodies
References
Super-multiplexvibrationalimaging.Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.αvIntegrinscombinewithLC3andatg5toregulateToll-likereceptorsignallinginBcells.Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.

Mechanicalsignalingcoordinatestheembryonicheartbeat.Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.

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