hypermol/Gelsolin (cytoplasmic) - 250µg/8304-02

Protein

Gelsolin (cytoplasmic)

Origin

smooth muscle, porcine

Molecular mass

80kDa

Protein description

Cytoplasmic gelsolin has a molecular mass of 80kDa and consists of six homologous subdomains (S1-S6). Gelsolin is a Ca⁺⁺-dependent lipid binding actin regulatory protein, possessing three different actin modulating activities: severing of F-actin, nucleation of actin polymerization and capping of filaments barbed end.Gelsolin has three actin binding sites, two high affinity Ca⁺⁺binding sites and two binding sites for PIP2.

Actin interaction

Ca++-sensitive actin capping, nucleating and severing protein.Kd actin-binding = 50nM (barbed end).

Form

Lyophilized, ready-to-use.

Quantity per unit

1x250 µg

Buffer

10mM Imidazole pH 7.0, 0.2mM DTT, 0.2mM EGTA, 2mM NaN₃and 1% disaccharides,when reconstituted with 250µlultrapure water to obtain a 1.0 mg/ml solution.

Purity &Activity

Purity: >90%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels.

Fragmentation activity:The fragmentation activity of gelsolin was determined by Ostwald viscometry at 25°C. The specific viscosity of F-actin (1mg/ml) is reduced by ~70% after addition of gelsolin at a molar ratio of 1:100. The gelsolin stock solution was used as described below containing 1% disaccharides.F-actin containing 100mM KCl, 10mM Imidazole pH 7.4, 1mM ATP was mixed with 0.2mM CaCl2 (final concentration) prior to the addition of gelsolin.

Purification notes

Protein concentration

Determinedby Biuret method with actin (skeletal muscle, rabbit) as reference.

Storage instructions

Gelsolin isstored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilizedproteinis kept on ice and should be used within 5 days. Storage in glycerol at -20°C results in a significant decrase of the activity, and has to determined individually.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

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