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| Description | Thisproductisnolongeravailable.Pleaseseebelowforourrecommendedalternativeproducts.
BatchNo. | Seeproductlabel | Unitsize | 100µg | Antigen | Asyntheticpeptide(QMQLEANADTSVEEESF)correspondingtoaregion(4-20)fromhumanDNArepairproteinRAD51homolog1.Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. | OtherNames | hRAD51;HsRAD51;RAD51;RAD51A;RECA; | Accession | Q06609RAD51_HUMAN; | Producedin | Rabbit | Purity | Affinitypurifiedonantigencolumn | Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0µg/mlisrecommendedforWB.HumanRAD51A(isoform1)hasapredictedlengthof339aminoacidsandaMWof37kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetectRAD51Ainformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. | Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. | Cross-reactivity | Human;rat;predictedtoreactwithmouseduetosequencehomology; | Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. | Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 | Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. | Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. | ExpiryDate | 12monthsafterpurchase | 新闻动态
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