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| Description | BDNFbelongstotheneurotrophinfamilyandpromotesthesurvivalofneuronalpopulationsthatarealllocatedeitherinthecentralnervoussystemordirectlyconnectedtoit.ItisamajorregulatorofsynaptictransmissionandplasticityatadultsynapsesinmanyregionsoftheCNS.TheversatilityofBDNFisemphasizedbyitscontributiontoarangeofadaptiveneuronalresponsesincludinglong-termpotentiation(LTP),long-termdepression(LTD),certainformsofshort-termsynapticplasticity,aswellashomeostaticregulationofintrinsicneuronalexcitABIlity.ThealterationsinBDNFexpressioninducedbyvariouskindsofbraininsultincludingstress,ischemia,seizureactivityandhypoglycemia,maycontributetosomepathologiessuchasdepression,epilepsy,Alzheimer"s,andParkinson"sdisease.MicrogliareleaseBDNFthatmaycontributetoneuroinflammationandneuropathicpain.SUBUNIT:Monomersandhomodimers.BindstoNTRK2/TRKB.SUBCELLULARLOCATION:Secretedprotein.POsttranslationmodification:ConvertedintomatureBDNFbyplasmin(PLG).SIMILARITY:BelongstotheNGF-betafamily. |
| Relatedproducts | Availableas100µg(2x50µg). |
| BatchNo. | Seeproductlabel. |
| Unitsize | 50µg. |
| Antigen | RecombinanthumanmatureBDNFexpressedinE.coli |
| AntibodyType | Mousemonoclonal. |
| Clone | 4C8 |
| OtherNames | Brain-derivedneurotrophicfactor;Abrineurin;proBDNF |
| Accession | BDNF_HUMAN |
| Producedin | Mouse |
| MolecularWeight | MatureBDNFdimerhasamolecularweightof28kDa.InWesternBlotting,matureBDNFmonomermigratesat14kDa. |
| Purity | ProteinGpurifiedmouseIgG. |
| Applications | ELISA:50-100ng/mL WesternBlotting(denaturingandreducing):0.2to2µg/mL.M-1744-50antibodydetects14kDamatureBDNFmonomerinhumanserumhumanandacidtreatedhumanandrodentwholetissuehomogenates.InsimpleTris-homogenatehumanbrainlysates,M-1744-50detectsmatureBDNFat14kDaaswellasanuncharacterizedbandat~50kDa.NoproBDNFbandisdetectedinoursamples,althoughM-1744-50willdetectproBDNFinpurifiedformonblots.Additionally,M-1744-50detectsanadditionalband18kDaBDNFisoformhumanserum.Forcelllysates,somecautionisadvisedasinourhandsM-1744-100failstodetectmatureBDNFonSH-SY5YorC6celllysatesbutdoesdetectmatureBDNFinneuronalcellculturelysates.Thereasonforthesedifferenceshasnotbeencharacterized.FordetectionofmatureBDNFincelllysatessuchasSH-SY5Ywerecommendaffinity-purifiedrabbitpolyclonalantibodytorhBDNF(R-1707-100),orrabbitpolyclonalantibodytoBDNFpeptide1-10(R-083-100,wholeserum;R-066-500,IgG). Immunofluorescence:1to4µg/mL,4%formaldehydefixationandpermeabilization.Stainingcanbeweakuntiloptimized. M-1744-50isnotrecommendedforFlowCytometryatthistime. Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Detectshuman,mouse,rat,guineapigBDNF.ExpectedtodetectBDNFfromotherspeciesduetosequencehomology. |
| BlastURL | Clickhere |
| Form | LyophilizedfromasolutioncontainingPBSpH7.4,3%trehalose,with0.1%sodiumazide. |
| Reconstitution | Spinvialbrieflybeforeopening.Reconstitutewith50µLsterilewatertoobtainaconcentrationof1mg/mL. |
| Storage | Storelyophilizedantibodyat-20°Cto-80°Cprotectedfrommoisture.Afterreconstitutiondivideantibodyintousefulaliquotsandkeepaliquotsat-20°Cto-80°Cforahigherstability.Workingaliquotscanbekeptat2-8°Cforupto1month.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchaseifunopened. |
| References | InstructionsforAcid-ExtractionofBrainTissue BDNFAntibodiesforWesternBlotting |
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