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| Description | This product is no longer available. Please see below for our recommended alternative products. |
| Unit size | 100 µg |
| Antigen Location | A synthetic peptide (QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL ) corresponding to a region (201-233) from human Tumor necrosis factor alpha. |
| Other Names | TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; TNF; TNFA; TNFSF2; |
| Accession | P01375 TNFA_HUMAN; |
| Produced in | Rabbit |
| Purity | Affinity purified on antigen column |
| Applications | Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1µg/ml is recommended for WB. Human TNF-alpha (precursor) has a predicted length of 233 residues . A concentration of 1 µg/ml is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Antigen retrieval is recommended Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity | The specificity of this antibody has been confirmed by WB and IHC against the antigen |
| Cross-reactivity | Human; mouse; rat; |
| Blast it | If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example. |
| Form | Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 added as preservative |
| Reconstitution | Reconstitute in 100 µl of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material. |
| Storage | At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles |
| Expiry Date | 12 months after purchase |
| References | Takeuchi H. et al. Tumor necrosis factor-alpha induces neurotoxicity via glutamate release from hemichannels of activated microglia in an autocrine manner. J Biol Chem. 2006 Jul 28;281(30):21362-8. |
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