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Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@biosensis.com| Description | TheratTNF-alphaKitisasandwichELISA.ThecaptureantibodyisamonoclonalratTNF-alphaantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.RattestsamplesandstandardsofknownTNF-alphaconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundTNF-alphaantibody.AbiotinylatedratTNF-alphapolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofTNF-alphapresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofratTNF-alphainsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | BatchNo. | Seeproductlabels | Antigen | Tumornecrosisfactoralpha(TNF-alpha)isacytokinethatbelongstothetumornecrosisfactor(TNF)superfamily.TNF-alphaismainlysecretedbymacrophages.TNF-alphaisinvolvedintheregulationofmanyprocessessuchas�cellproliferation,differentiationandapoptosisandhasbeenimplicatedinavarietyofdiseasesincludingcancer.TNF-alphaisexpressedasasingle-passtypeIImembraneprotein.Asoluble,secretedformisderivedfromthemembraneformbyproteolyticprocessing. | OtherNames | TNF-alpha;Tumornecrosisfactorligandsuperfamilymember2;TNF-a;Cachectin;TNF;TNFA;TNFSF2; | Accession | P16599TNFA_RAT; | Specificity | RatTNF-alpha | Storage | Storeat4°C | Kitcomponents | TheELISAkitboxcontains1x96pre-coatedstripplate,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 15.6pg/ml-1,000pg/mlforbodyfluids,tissuelysatesorcellculturesupernates.7.8pg/ml-500pg/mlforratsera. | Sensitivity | < 1 pg/ml | Kitprotocol |  BEK-2101-1P_as_at_Jun2017.pdf新闻动态
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