企业QQ
官方微信
| Description | FUNCTION:Target-derivedsurvivalfactorforperipheralsensorysympatheticneurons.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Highestlevelsinprostate,lowerlevelsinthymus,placenta,andskeletalmuscle.Expressedinembryonicandadulttissues.SIMILARITY:BelongstotheNGF-betafamily. |
| BatchNo. | Seeproductlabel |
| Unitsize | 500µg |
| Antigen | RecombinanthumanNT4 |
| OtherNames | Neurotrophin-5;NT-5;Neutrophicfactor5;Neurotrophin-4;NT-4;Neutrophicfactor4;NTF5;NTF4 |
| Accession | NT5_HUMAN |
| Producedin | Rabbit |
| Purity | ProteinGpurifiedIgG |
| Applications | IHC,ELISA(1site),WesternBlot,ICC,inhibitionofbiologicalactivityinvitro/invivo.Recommendedtobeusedataconcentrationof1-10µg/mlforimmunohistochemistry,ELISA,ICCandWesternblotandinhibitionofbiologicalactivityinvitro.Useneatforinvivostudiesat2-10µg/ml(ED50).NotethattheconcentrationofNT4isgenerallylowinmosttissuesnevertheless,neonataltestesofratcanbeusedasagoodpositivecontrol.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Lessthan1%cross-reactivityagainstNGF,recombinanthumanBDNFand5%toNT3hasbeenshownbydotblot. |
| Cross-reactivity | KnowntoreactwithNT4fromratandhuman,mouseandmonkey. |
| Blastit | IfyouwouldliketoseethesharedidentitybetweendifferentspeciesorotherproteinsfollowthelinkinAccessionfield,selectthenthesequence(makesurethatyouareselectingthesequencethatyouareinterestedin,asthesequencemaybetheprecursorratherthanthematureproteinforexample)andcopyandpasteitHEREandblast/formatit. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein500µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstability,andat4ºCwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability. |
| ExpiryDate | 12monthsafterpurchase |
| References | 1.Lodouichi(2000)J.Neuroscie.20(6):2155 2.Zhangetal.(1999)JNeurosci.Meths.89(1):69 |