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| Description | FUNCTION:Functionsasacellsurfacereceptorandperformsphysiologicalfunctionsonthesurfaceofneuronsrelevanttoneuritegrowth,neuronaladhesionandaxonogenesis.Involvedincellmobilityandtranscriptionregulationthroughprotein-proteininteractions.CanpromotetranscriptionactivationthroughbindingtoAPBB1/Tip60andinhibitNotchsignalingthroughinteractionwithNumb.Couplestoapoptosis-inducingpathwayssuchasthosemediatedbyG(O)andJIP.InhibitsG(o)alphaATPaseactivity.ActsasakinesinImembranereceptor,mediatingtheaxonaltransportofbeta-secretaseandpresenilin1.Maybeinvolvedincopperhomeostasis/oxidativestressthroughcopperionreduction.CanregulateneuriteoutgrowththroughbindingtocomponentsoftheextracellularmatrixsuchasheparinandcollagenIandIV.FUNCTION:Beta-amyloidpeptidesarelipophilicmetalchelatorswithmetal-reducingactivity.Bindtransientmetalssuchascopper,zincandiron.Ratandmousebeta-amyloidpeptidesbindonlyweaklytransientmetalsandhavelittlereducingactivityduetosubstitutionsoftransientmetalchelatingresidues.Beta-APP42mayactivatemononuclearphagocytesinthebrainandelicitinflammatoryresponses.PromotesbothtauaggregationandTPKII-mediatedphosphorylation(Bysimilarity).FUNCTION:Thegamma-CTFpeptidesaswellasthecaspase-cleavedpeptides,includingC31,arepotentenhancersofneuronalapoptosis.SUBUNIT:Binds,viaitsC-terminus,tothePIDdomainofseveralcytoplasmicproteins,includingAPBBfamilymembers,theAPBAfamily,MAPK8IP1,SHC1,NumbandDab1.BindingtoDab1inhibitsitsserinephosphorylation.AlsointeractswithGPCR-likeproteinBPP,FPRL1,APPBP1,IB1,KNS2(viaitsTPRdomains),APPBP2(viaBaSS)andDDB1.Invitro,itbindsMAPTviatheMT-bindingdomains.AssociateswithmicrotubulesinthepresenceofATPandinakinesin-dependentmanner.Interacts,throughaC-terminaldomain,withGNAO1.Amyloidbeta-42bindsCHRNA7inhippocampalneurons.Beta-amyloidassociateswithHADH2.TISSUESPECIFICITY:differentisoformsindifferenttissues:kidney.brain.liver.hippocampus,substanianigraparscompactaandcerebellum.Inthecerebellum,alltheisoformsareabundantlyexpressedinPurkinjecells. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Asyntheticpeptide(HMNVQNGKWESDPSGTKTC,aa:44-62)aspartofmouseAPPisoformAconjugatedtotheimmunogenicproteinBlueCarrierProtein |
| OtherNames | AmyloidbetaA4protein;ABPP;Alzheimerdiseaseamyloidproteinhomolog;Amyloidogenicglycoprotein;AG |
| Accession | APP_MOUSE |
| Producedin | Rabbit |
| Purity | Wholeserum |
| Applications | IHC.Recommendedtobeusedatadilutionof1:500to1:3000forimmunohistochemistry.Thisantiserumhasnotyetbeentestedforwesternblot.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | SpecificityforAPPwasconfirmedbyIHC. |
| Cross-reactivity | ThisantiserumisknowntoreactwithratAPP.Reactivitywithotherspecieshavenotyetbeentested. |
| Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedIDbetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstability,andat4ºCwithanappropriateantibacterialagent.Glycerol(1:)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
| References | 1.Wilson,C.A.,etal.,J.Neurosci.Res.74:361-369(2003). 2.Andreasen,N.,etal.,WorldJ.Biol.Psychiatry4:147-155(2003). 3.Guenette,S.Y.NeuromolecularMed.4:147-160(2003). |