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| Description | HeatShockProtein40(HSP40)isachaperoneprotein.Heatstresstriggerstherapid translocationofHSP40fromthecytoplasmtothenucleus.HSP40interactswithHSP70andcanstimulateitsATPaseactivity. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(EFEVIFPERIPQTSRT)correspondingtoaregion(317-332)fromhumanHeatShockProtein40(HSP40).Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. |
| OtherNames | Heatshock40kDaprotein1;Heatshockprotein40;HSP40;DnaJproteinhomolog1;HDJ-1;DNAJB1;DNAJ1;HDJ1;HSPF1; |
| Accession | P25685DNJB1_HUMAN; |
| Producedin | Rabbit |
| Purity | Affinitypurifiedonantigencolumn |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0-2.0µg/mlisrecommendedforWB.HumanHSP40hasapredictedlengthof340residuesandMWof38kDa.Aconcentrationof0.5-1.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Antigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;mouse;rat; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
| Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles |
| ExpiryDate | 12monthsafterpurchase |