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| Description | Bcl-2-likeprotein1isasingle-passmembraneproteinbelongingtotheBcl-2familyofproteinsthatcanactasanti-orpro-apoptoticregulators.Atleast3isoformsofBcl-2-likeprotein1areproducedbyalternativesplicing.IsoformBcl-X(S)isexpressedathighlevelsincellsthatundergoahighrateofturnoversuchasdevelopinglymphocytesandisanactivatorofapoptosis. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(YQSFEQDTFVE)correspondingtoaregion(120-130)fromhumanBcl-2-likeprotein1isoformBcl-X(S).Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. |
| OtherNames | Bcl-2-likeprotein1;Bcl2-L-1;ApoptosisregulatorBcl-X;BCL2L1;BCLX;bcl-xS;bcl-xbeta; |
| Accession | Q07817B2CL1_HUMAN; |
| Producedin | Rabbit |
| Purity | Affinitypurifiedonantigencolumn |
| Applications | WesternBlotting(WB).Aconcentrationof1.0µg/mlisrecommendedforWB.HumanBcl-2-likeprotein1isoformBcl-X(S)hasapredictedlengthof170aminoacidsandMWof19kDa.Aconcentrationof2.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;rat;predictedtoreactwithmouseduetosequencehomology; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
| Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |