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| Description | Neurofilamentscontainthreeintermediatefilamentproteins:light(68kDa),medium(160kDa)andheavy(200kDa).Neurofilamentheavy(NF200orNF-H)isphosphorylatedanditisthoughtthatthisresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.ThisantibodybindsprimarilytothephosphorylatedaxonalformsofNF-H. |
| BatchNo. | Seeproductlabel |
| Unitsize | 50µl |
| Antigen | PurifiedbovineNeurofilamentHeavy(NF-H) |
| Isotype | IgY |
| OtherNames | NF-200;NF200;NF-H;NEFH;N52;Neurofilamentheavypolypeptide;NeurofilamenttripletHprotein;200kDaneurofilamentprotein;KIAA0845;NFH; |
| Accession | P12036NFH_HUMAN; |
| Producedin | Chicken |
| Applications | WesternBlotting(WB),Immunocytochemistry(IC)andELISA.SuggesteddilutionforWBis1:100,000-1,000,000.SuggesteddilutionforICis1:50,000-100,000.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThisantibodyreactswithphosphorylatedNF-Handisseenasabandofapprox200kDainWB.RefertopublicationbyShawetal(2005)fortheuseofthisantibodyinanELISAtodetectNF-H. |
| AntibodyAgainst | NeurofilamentHeavyphosphorylated |
| Cross-reactivity | Hu,Rat,Ms,Fel,Chk.Predictedtoreactwithothermammaliantissuesduetosequencehomology. |
| BlastURL | Clickhere |
| Form | Lyophilisedwith5%trehalose |
| Appearance | Whitepowder |
| Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | 1.JarjourA.A.etal(2007)Maintenanceofaxo-oligodendroglialparanodaljunctionsrequiresDCCandnetrin-1.JNeurosci.2008Oct22;28(43):11003-14. 2.PearseD.D.etal(2007)TransplantationofSchwanncellsand/orolfactoryensheathinggliaintothecontusedspinalcord:Survival,migration,axonassociation,andfunctionalrecovery.Glia.2007Jul;55(9):976-1000. 3.ShawG.etal(2005)HyperphosphorylatedneurofilamentNF-HisaserumbioMarkerofaxonalinjury.BiochemBiophysResCommun.2005Nov4;336(4):1268-77. |