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Finda1platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@biosensis.com| Description | TheratInterleukin1beta(IL-1b)KitisasandwichELISA.ThecaptureantibodyisamonoclonalmouseIL-1bantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.RattestsamplesandstandardsofknownIL-1bconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundIL-1bantibody.AbiotinylatedIL-1bpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-1bpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofratIL-1binsamplessuchassera,plasma(heparin,EDTA),tissuehomogenates,cellculturesupernatantsandcelllysates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | BatchNo. | Seeproductlabels | OtherNames | Interleukin-1b;IL-1b;IL1b; | Accession | Q5BKB0Q5BKB0_RAT | Specificity | RatIL-1b | Storage | Storeat4˚Cfor6months,at-20˚Cfor12months.Avoidmultiplefreeze-thawcycles. | Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 31.2pg/ml-2000pg/ml | Sensitivity | < 1 pg/ml, determined as concentration at 2 x SD of blank (n = 20). | Kitprotocol |  BEK-2309-2P_as_as_May2017.pdf新闻动态
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